Practice Test


1) Tissue culture technique has been biotechnologically successful in production of:


2) Estimated number of antibiotics produced by Streptomyces griseus is:


3) The first relatively pure enzyme was prepared by:


4) Shikonin is the name of:


5) Yeast is a good source of:


6) Agar agar is obtained from:


7) The conditions which favour fermentation are:


8) Penicillin is obtained from:


9) The vinegar means:


10) Penicillin was first discovered by:


11) Yeast is used in the production of:


12) First antibiotic isolated was:


13) Streptomycin was first isolated in 1944-45 by:


14) In the production of leavened bread the following is used:


15) Most of the micro-organisms which produce antibiotics live in the soil because:


16) Streptomycin is produced by:


17) Who was the first to isolate streptomycin?


18) Chloramphenicol and erythyromycin (broad spectrum antibiotics) are produced by:


19) Chloromycetin is produced by:


20) Terramycin is obtained from:


21) Enzymes can be immobilised by:


22) The number of enzymes known today is approximately:


23) Who of the following scientists showed that Saccharomyces cerevisiae caused fermentation products such as beer and buttermilk?


24) Which enzymes are used for converting corn starch into high fructose syrup?


25) A bioreactor refers to:


26) Germinating barley seeds are employed in the preparation of:


27) Cells obtained from cancerous tumours are known as:


28) As in Hepatitis B vaccine:


29) Rennet enzyme was purified by:


30) The enzyme diastase was identified by:


31) Which enzymes are used for manufacturing detergents?


32) The enzyme TPA is used to:


33) Hybridomas are employed for:


34) The method of growing microorganisms as a thin layer on nutrient medium is known as:


35) Pasteurization makes foodstuffs free from:


36) Number of antibiotics isolated till today is:


37) Tissue plasminogen activator is:


38) For rapid production of alcohol, immobilised yeast cells are kept in:


39) Dextran is used in:


40) Which of these participates in the manufacturing of dextrans?


41) Steroids are used in:


42) Utility of fungi for steroid conversion was demonstrated by:


43) Humulin is:


44) Humulin was launched by:


45) The micro-organism involved in making bread is:


46) Which of the following organic acids was produced by fermentation?


47) Most of the Eubacteriale antibiotics come from:


48) Restriction enzyme was discovered by:


49) Hybridomas are a result of fusion of:


50) Kohler and Milstein developed Biotechnology for the production of:


51) Enzymes used in detergents are:


52) Torulopsis utilis is:


53) Antibiotics inhibit the growth of or destroy:


54) If all the bacteria and fungi of the world are destroyed then:


55) Streptomyces venezuelae is used to obtain:


56) Citric acid is produced by:


57) The first commercial example of enzyme modification of a protein for human use is:


58) Which of the following is used for the diagnosis of disease caused by closely related pathogen?


59) Utilization of living organisms/systems/processes for producing materials useful to human society is known as:


60) Biotechnology utilizes new organisms developed from present organisms utilizing:


61) Which of the following is not concerned with biotechnology?


62) Which of these is used in biotechnology:


63) Which of the following is used for manufacture of cosmetics?


64) The technique by which virus detection can be make both in plants and animals is known as:


65) Streptokinase (TPA) helps in:


66) The immobilization of enzyme is achieved by:


67) Interferons are:


68) Magic bullets are:


69) Cheese contains:


70) The hybridoma technique was developed by:


71) Which one of the following micro-organisms is used for production of citric acid in industries?


72) Important objective of biotechnology in agriculture sector is:


73) The main technique involved in agricultural biotechnology is called:


74) The new strain of bacteria produced by biotechnology in alcohol industry is:


75) The basis of DNA fingerprinting is:


76) Genetically engineered bacteria have been used in commercial production of:


77) Which of the following correctly defines a transgenic animal?


78) Which one of the following is not true about antibiotics?


79) Rennin used in cheese industry is:


80) The organism used for alcoholic fermentation is:


81) A cell coded protein that is formed in response to infection with most animal viruses is called:


82) Which of the following organelles is related with genetic engineering?


83) A chemical susbtance derived from a living source that has the capacity to inhibit growth or to destroy microbes is called:


84) The Central Drug Research Institute (CDRI) is situated in:


85) Wine and Beer are produced directly by fermentation Brandy and Whisky require both fermentation and distillation because:


86) The antibiotics obtained from Streptomyces are:


87) Human insulin has 51 amino acids in two chains. Mark the correct statement:


88) During "gene cloning" which is called as "gene taxi"?


89) What does Bt stands for the popular crop Bt cotton?


90) Name the drug used in cancer treatment produced by using biotechnology:


91) Use of biology in industrial process and for improving quality of life is called:


92) What is the reason of formation of embryoid from pollen grain in tissue culture medium?


93) The technique of obtaining large number of plantlets by tissue culture method is called:


94) Somaclonal variation can be obtained by:


95) Cultivation of Bt cotton has been much in the news. The prefix Bt means:


96) In transgenics, expression of transgene in target tissue is determined by:


97) The Ti plasmid is often used for making transgenic plants. This plasmid is found in:


98) The first antibiotic was discovered by


99) Colchicine brings about:


100) A cybrid is a hybrid carrying:


101) Biogas produced by anaerobic decomposition used in combustion is mainly due to presence of:


102) Which of the following is not an antibiotic?


103) Which raw material is used in fermentation process of making beer?


104) Which one of the following is the correctly matched pair of a product and the microorganism responsisble for it?


105) Hybridoma technology has been successfully used in:


106) India's wheat yield revolution in the 1960s was possible primarily due to:


107) A self-fertilizing trihybrid plant forms:


108) Bacillus thuringiensis (Bt) strains have been used for designing novel:


109) Which of the following is generally used for induced mutagenesis in crop plants?


110) An institution where valuable plant material likely to become irretrievably lost in the wild or in cultivation is preserved in a viable condition is known as:


111) Hirudin is:


112) Golden rice is a promising transgenic crop, when released for cultivation, it will help in:


113) Which antibiotic inhibits interaction between tRNA and mRNA during bacterial protein synthesis?


114) Select the wrong statement:


115) A transgenic food crop which may help in solving the problem of night blindness in developing countries is:


116) Main objective of production/use of herbicide resistant GM crop is to:


117) Human insulin is being commercially produced from a transgenic species of:


118) What is antisense technology?


119) The process of RNA interference has been used in the development of plant resistant to:


120) A plant part that is excised from its original location and used for initiating a culture is termed:


121) Growth regulator commonly used in plant tissue culture is/are:


122) Shoot regeneration is promoted by:


123) Which of the following is used to obtain single cell protein used as food or feed?


124) A West African plant produces a protein called Pentadiplandra brazzeana which is about 2000 times as sweet as sugar:


125) Which of the following statement is incorrect?


126) Which transgenic is used in organism in production of hirudin?


127) Protoplast of plant cell is obtained by working:


128) Ringworm of scalp is caused by:


129) An enzyme used in cheese making is:


130) Biopiracy is related to which of the following?


131) Which of the following is used in biowar?


132) Which of the following is inculded in biopesticide?


133) Which of the following can be controlled by using biopesticides?


134) Biofertilizers include:


135) Which of the following risks are associated with genetically modified food?
I. Toxicity
II. Allergic reaction
III. Antibiotic resistance in microorganisms present in alimentary canal


136) Embryo culture is used for:


137) A transgene expression can achieve which of the following?


138) The plant cells without cell wall is called:


139) Biotechnology deals with industrial scale production of biopharmaceuticals and biologicals using genetically modified


140) The application of biotechnology includes all except


141) Which of the following is not included under the application of biotechnology?


142) Three critical research areas of biotechnology are -
I. Providing the best catalyst in the form of improved organism usually a microbe or pure enzyme
II. Multiple Ovulation Transfer Technology (MOET)
III. Creating optimal conditions through engineering for a catalyst to act
IV. Downstream processing technologies


143) Which of the following is for increasing food production?


144) The crops having cry-genes need


145) Cry-genes have been introduced in


146) First transgenic crop is


147) Bacillus thuringiensis is a bacterium of


148) Which is not a transgenic plant?


149) Bt toxin is


150) Bt is resistant to


151) Genetically modified tobacco plant with Bt gene is resistant to


152) I. Recombinant DNA technology is used to improve crop plants by increasing their productivity, by making them more nutritious and by developing disease resistant.
II. Bt cotton is resistant to bollworm infestation.
III. Bacillus thuringiensis form cry protein during any phase of their growth.
IV. Bacillus thuringiensis is not harmed by self Cry protein because of its occurrence as protoxin (inactive).
V. Protoxin Cry protein is changed into active Cry protein in the stomach of insects due to alkaline pH in stomach.


153) Bt toxin is


154) Bt gene produces protein toxin to insect larvae is


155) A plant expressing a gene from another organisms is


156) Introduction of transgene will result in


157) Bt toxin is harmful to insects like


158) How does Bt toxin kill the larvae of certain insects?


159) Bt toxin genes are isolated from Bacillus thuringiensis and incorporated into crop plants making them insecticidal. The choice of genes depend upon


160) Which of the following is correct?


161) Which of the following is a correct statement?


162) Bt cotton farming has shown good result in


163) Several nematodes parasitise


164) When DNA is transcribed into mRNA, usually the mRNA remains single-stranded, but in some cases an RNA can be made that is complementary to the mRNA. This is called _______ and its main function is to _______.


165) Which of the following is the source of complementary strand in mRNA silencing


166) Antisense RNA is


167) Which of the following nematode infects the roots of tobacco plants and causes a great reduction in yield?


168) The RNAi stands for


169) mRNA silencing is called


170) Fill up the blanks -
At present, about _________ recombinant therapeutics have been approved for human use the world over. In India ______ of these are presently being marketed.


171) The first human drug made using recombinant DNA technology was


172) Which of the following statements is false?
I. Insulin was originally extracted from pancreas of slaughtered pigs and cattle
II. Animal insulin is difficult to obtain
III. Animal insulin is identical to human insulin
IV. Non human insulin caused some patients to develop allergy
V. Recombinant DNA allowed scientists to insert a human insulin gene into a bacterial expression vector
VI. Recombinant insulin is actually obtained from E. coli bacterial cell


173) RNA interference (RNAi) technique has been devised to protect the plants from nematode is silenced by ________ produced by the host plant.


174) Which of the following peptide chain is removed during maturation of pro-insulin into insulin?


175) Eli Lilly, an American company prepared two DNA sequences corresponding to A and B, chains of human insulin and introduced them in plasmids of E. coli to produce insulin chains. Chains A and B were prepared separately, extracted and combined by creating


176) Which step proved to be the main challenging obstacle in the production of human insulin by genetic engineering?


177) What is the disadvantage of using porcine insulin (from pig) in diabetic patients?


178) Why insulin not administered orally to diabetic patient?


179) The first clinical gene therapy was given in 1990 to a 4 years old girl with which of the following enzyme deficiency?


180) Which of the following could be a permanent cure for treatment of severe combined immunodeficiency (SCID)


181) Why is repeated transfusions of genetically engineered cells required in SCID patients?


182) Human gene therapy requires


183) When a patient with defective DNA was treated, which of the following steps was performed for gene therapy?
I. Lymphocytes were obtained from the patients
II. Lymphocytes are transferred to culture dishes
III. Lymphocytes were transfected with normal ADA genes
IV. The transfected cells are returned to the patients


184) Which technique would you expect to be completely curative in SCID?


185) Which of the following is a benefit to having insulin produced by biotechnology?


186) Following are the steps in one type of gene therapy -
I. Inject engineered cells into patients bone marrow
II. Viral DNA carring the normal allele inserts into chromosome
III. Let retrovirus infect bone marrow cells that have removed from patient and cultured
IV. Insert RNA version of normal allele into retrovirus
Which of the following is correct sequence of steps


187) Which of the following is correct about ADA?


188) "Silencing" of mRNA molecule in order to control the production of a harmful protein has been used in protection of plants from


189) For effective treatment of a disease


190) Which of the following techniques serve the purpose of early diagnosis?
I. R-DNA technology
II. PCR
III. ELISA
IV. Convential method of diagnosis (serum, urine analysis etc.)


191) A doctor while operating on an HIV + patient accidentally cut himself with a scalpel. He comes to you, suspecting himself to have contracted the virus which test will you advise him to rule out/confirm his suspicion?


192) In order for gene therapy to be most effective, genes should be inserted in


193) A nuclei acid probe might be used to


194) Which of the following is a critically important tool used in experiments involving DNA hybridization?


195) A sample of DNA from a person suspected of having sickle-cell anaemia is subjected to DNA hybridization using two probes. One that binds to the normal allele and another that binds to the sickle-cell allele. If both probes bind to the DNA, this individual


196) PCR is used to


197) Which of the following is based upon the principle of antigen antibody interaction?


198) DNA hybridization technique is based on all of the following properties of DNA except


199) Which one of the following genes is defective in patients suffering from severe combined immuno-deficiency syndrome (SCID)?


200) A functional ADA cDNA can be introduced into cells of the patients receiving gene therapy by using vector constituted by


201) Deliberate alteration of genome for treatment of disease is called


202) Which one is correct?


203) What might be an advantage of beginning gene therapy prior to birth?


204) Which of the following is correct?


205) A correct pair of characteristics of molecular probe is -
A. Very long molecule
B. Double stranded
C. Single stranded DNA or RNA
D. Complementary to part of desired gene


206) The DNA probe, 3' - GGCTTA, will hybridize with which of the following?


207) Restriction enzymes were first discovered with observation that


208) Following are the steps of southern blot procedure -
I. Autoradiography
II. Hybridization with radioactivity nucleic acid (probe)
III. Blotting
IV. DNA fragments are treated to make them single stranded
V. Electrophoresis
VI. Clearing of DNA of restriction endonuclease
VII. Isolation of DNA from sample
The correct sequence is


209) Which method of cellular defence is common to all eukaryotic organisms?


210) The technique by which "Dolly" the sheep was obtained is called


211) Transgenic animals are those which have


212) Transgenic rats, rabbits, pigs, sheep, cows and fish have produced although over 95 percent of all existing transgenic animals are


213) Transgenic animals are produced for which of the following purposes?
I. To study how gene are regulated and how they affect the normal functions of body and its development
II. To study of diseases
III. To obtain useful biological product
IV. To test vaccine safety and chemical safety


214) Bioaugmentations is


215) In which disease the advancement of genetic engineering has still not been used as clinical cure


216) Which of the following transgenic protein product has been used to treat emphysema


217) "Rosie" a transgenic cow known to produce a type of milk which has all the following characteristics except


218) Rosie was produced in year


219) GMO/transgenic animal are used in testing safety of polio vaccine before they are used on human?


220) To test the vaccine safety before its use on human, transgenic ______ are used to test. If successful, _______ are used to test the safety of vaccine


221) Which of the following is correct?


222) "Pharming" is a term that describes


223) Which of the following statements is correct?


224) Introduction of genetically modified food is not desirable because


225) How many varieties of rice has been estimated to be present in India?


226) The use of bioresources by multinational companies and other organizations without proper authorization from the countries and people concerned without compensatory payment is called


227) Which variety of rice was patented by a U.S. company even though the highest number of varieties of this rice is found in India?


228) Which step of Government of India has taken to cater to the requirement of patent terms and other emergency provisions in this regard?


229) Which Indian plants have either been either patented or attempts have been made to patent them by western nations for their commercial use?


230) A transgene expression can achieve which of the following?


231) Biopiracy is related to which of the following


232) Which is being synthesized by genetic engineering?


233) How many recombinant therapeutics have been approved for human use the world over?


234) In some children, ADA deficiency can be cured by


235) Bt toxin is produced by


236) Today, transgenic models exist for many human diseases which includes -
A. Cancer
B. Cystic fibrosis
C. Rheumatoid arthritis
D. Alzhiemer's disease


237) It is now possible to breed plants and animals with desired characters through


238) Main objective of producing herbicide resistant GM crops is


239) Addition of foreign gene into a crop is


240) Transgenic bacteria are being used for producing


241) Transgenic Golden Rice is enriched with high


242) A transgenic crop has genes for


243) Reagent used in ELISA test is


244) A genetically engineered microorganism used successfully in bioremediation of oil spills is a species of


245) In Bt cotton, a transgenic plant, Bt refers to


246) Which of the following cannot be patented


247) Technique of production of monoclonal antibodies was developed by


248) Genetic modification has -
A. made crops more tolerant to abiotic stresses.
B. reduced reliance on chemical pesticides.
C. increased efficiency of minerals use by plants.
D. enhanced nutritional value of food.


249) Which of the following statement is not true.


250) Which of the following statements is/are true about genetically engineered insulin?
A. its name is humulin
B. it was manufactured by American firm Eli Lilly
C. it was launched on 5 July 1983
D. it is produced by the fermentation of appropriate recombinant E. coli clones


251) The first clinical gene therapy was given for the deficiency of which of the following enzymes?


252) The actual process of splicing DNA fragments into plasmids takes place in


253) Which Indian plants have either been patented or attempts have been made to patent them by western nations for their commercial uses.
A. Basmati rice
B. Turmeric
C. Neem
D. None


254) The decisions regarding the validity of genetic modification research and the safety of introducing genetically modified organisms for public services in India is taken by


255) The process of RNA interference takes place in all eukaryotic organisms as a method of cellular defense which -
A. Complementary dsRNA
B. Retroposons
C. Single Stranded DNA
D. Protein


256) Which of following is/are correct?
A. gene therapy has been tested on a large number of patients with a wider variety of inherited genetic disorders, and in numerous cases it has produced a complete cure.
B. genetic engineering has been used to mass produce insuline for diabetes.
C. DNA hybridization is the base pairing of DNA from two different sources.
D. genetic engineering is a technique of plant breeding.


257) Genetic engineering can be used to -
A. alter the performance of genetically modified organism.
B. produce multiple copies of a desired DNA sequence.
C. Generate many copies of specific genes.
D. Enhance the production of a specific gene product.


258) Go through the statements.
A. During the processing of the prohormone "proinsulin" into the mature "insulin", C-peptide is removed from proinuslin.
B. In hydridoma technology, B-cells are fused with myeloma cells.
C. Genetic engineering has been successfully used for producing transgenic mice for testing safety of polio vaccin before use in humans.
D. Some of the characteristics of Bt cotton are high yield and resistance to bollworms.
E. An improved variety of transgenic basmati rice gives high yield and is rich in vitamin A.
F. 'Gray biotechnolgoy' is referred to industrial process.
G. The polymerase chain reaction is a technique that is used for in vivo replication of DNA.
How many statement is wrong?


259) Which of the following is/are product(s) of genetic engineering -
A. Humulin
B. Hepatitis B vaccine prepared by yeast
C. Golden rice
D. Bt cotton


260) Select the correct statement(s)


261) Tobacco plants resistant to a nematode have been developed by the introduction of DNA that produced (in the host cells)


262) The first clinical gene therapy was given for treating


263) Consumption of which one of the following foods can prevent the kind of blindness associated with vitamin 'A' deficiency?


264) Molecular probes used for identification of recombinant clone carrying the desired DNA insert can be -
A. denatured double stranded DNA probes
B. double stranded RNA probes
C. protein probes
D. single stranded DNA probes


265) Plants, bacteria, fungi and animals whose genes have been altered by manipulation are called


266) GM plants have been useful in increasing


267) Proteins produced by Bacillus thuringiensis kill


268) Which one is incorrect?


269) Which is incorrect?


270) What is correct about insulin?


271) Genetically engineered human insulin is manufactured by the use of which of the following microorganisms?


272) The first hormone produced artificially by culturing bacteria was


273) Which enzyme is crucial for proper functioning of the immune system?


274) Which statement is incorrect about gene therapy?


275) PCR is routinely used to detect


276) A genetically manipulated organism containing in its genome one or more inserted genes of another species is called


277) It is now possible to breed plants and animals with desired characters through


278) Use of bioresources of other nations without proper authorization of the countries concerned is called


279) Rules of conduct that may be used to regulate our activities in relation to the biological world is called


280) Which one is not correct?


281) Detection of a clone is done by


282) Bt cotton is resistant to


283) Plants are more rapidly manipulated by genetic engineering than animal as


284) Terminator gene


285) Interferon is a glycoprotein which is


286) Which statement is correct?
A. Somaclonal variations are the variations observed in plants regenerated from somatic cultures.
B. A transgenic crop is one that contains and expresses a transgene.
C. Chloromycetin is obtained from Penicillium.
D. Bioprospecting is the process of discovery and commercialization of new products based on biological resources.


287) Which of the following is associated with transgenic animals?
A. Vaccine safety
B. Chemical safety
C. Biopiracy
D. Gene therapy


288) Interferon is -
A. antimalarial
B. antifungal
C. antiviral
D. anticancer


289) Humulin is -
A. an antibiotic
B. human insulin
C. antidiabetic
D. antifungal


290) Which of the following is related with gene therapy?
A. Enzyme replacement therapy
B. Bone marrow transplantation
C. Hybridoma technique
D. Hepatitis


291) The true statement about Genetic Engineering Approval committee (GEAC) is


292) In hybridization


293) DNA probes are used to


294) For DNA probes to hybridize


295) Statement - I : Non-homologous DNA will attach to a DNA probe.
Statement II : At high temperature, two complementary DNA strands will anneal.


296) The term humulin is associated with


297) In vitroclonal propagation in plants is characterized by


298) Insect pest resistant Bt-cotton plant was developed using


299) Human insulin is being commercially produced from


300) Find out the incorrect statement for transgenic animals


301) Go through the following statements.
I. Human insulin is being commercially produced from a transgenic species of Escherichia coli.
II. The genetically modified Bacillus thuringiensis is used as biopesticide on the commercial scale.
III. Human protein, alpha-1-antitrypsin is used to treat emphysema.
IV. The first transgenic cow, Rosie produced alpha lactalbumin, enriched milk.
V. Bt toxin genes cry I Ac control the corn borer.
Select the wrong statement(s)


302) The microbial bicontrol agent for butterfly caterpillar is


303) The transgenic animals are generally produced for all the following needs except


304) Which one of the following statements is wrong in relation to transgenic Bt cotton plant?


305) Which body of the Government of Indian regulates GM research and safety of introducing GM organisms for public services?


306) The crops engineered for glyphosate are resistant/tolerant to


307) Golden rice is genetically modified crop plant where the incorporated gene is meant for biosynthesis of


308) If any protein encoding gene is expressed in a heterologous host then protein is known as


309) The two polypeptides of human insulin are linked together by


310) Which part of the tobacco plant is infected by Meloidogyne incognita?


311) Which kind of therapy was given in 1990 to a four year old girl with adenosine deaminase (ADA) deficiency?


312) The first nif genes were isolated from


313) Manipulation of DNA in genetic engineering
became possible due to the discovery of


314) ELISA is used to detect viruses where the
key reagent is


315) Restriction endonucleases


316) In transgenics, expression of transgene in
target tissue is determined by


317) Restriction endonuclease


318) Gel electrophoresis is used for


319) The linking of antibiotic resistance gene
with the plasmid vector became possible
with


320) Polyethylene glycol method is used for


321) Which one of the following is used as vector
for cloning into higher organisms?


322) DNA or RNA segment tagged with a
radioactive molecule is called


323) Restriction endonucleases are enzymes
which


324) Stirred-tank bioreactors have been designed
for


325) Which of the following are used in gene
cloning ?


326) In genetic engineering, a DNA segment
(gene) of interexis transferred to the host
cell through a vector. Consider a following
four agents (i-iv) in this regard and select
the correct option about which one or more
of these can be used is vector/vectors
(i) Bacterium (ii) Plasmid
(iii) Plasmodium (iv) Bacteriophage


327) Given below is a sample of a portion of
DNAstrand giving the base sequence on the
opposite strands. What is so specie shown
in it ?
5’___GAATTC____ 3’
3’____CTTAAG ____ 5’


328) There is a restriction endonuclease called
Eco R I. What does “co” part in it stand for?


329) Agarose extracted from sea weeds is used
in


330) Which one of the following techniques made
it possible to genetically engineer living
organisms ?


331) PCR and restriction fragment length
polymorphism are the methods for


332) A singe strand of nucleic acid tagged with
a radioactive molecule is called


333) Which one of the following is a case of
wrong matching ?


334) Which one is a true statement regarding
DNA polymerase used in PCR ?


335) For transformation, micro-particles coated
with DNA to be bombarded with gene gun
are made up of


336) Biolistics (gene-gun) is suitable for


337) In genetic engineering, the antibiotics are
used


338) Which one of the following represents a
palindromic sequence in DNA ?


339) Dough kept overnight in warm weather
becomes soft a spongy because of


340) The Ti plasmid, is often used for making
transgenic roocs. The plasmid is found in


341) Golden rice is a transgenic crop of the future
sac me improved trait


342) Bacillus thuringiensis (Bt) strains have been
used for designing novel


343) Production of ahumanprotein in bacteria by
genetic engineering is possible because


344) Golden ride is a prortusing transgenic crop.
When released cultivation, it will help in


345) Two microbes found to be very useful in
genetic engineering are


346) What is antisense technology ?


347) Cry I endotoxins obtained from Bacillus thuringiensis are effective against


348) A transgenic food crap which may help in
solving the problem of night blindness in
developing countries is


349) Main objective of production/use of
herbicide resistant crops is to


350) Human insulin is being commercially ?


351) Which one of the following is commonly
used in transfer of Foreign DNA into crop
plants?


352) What is true about Bt toxin ?


353) Which one of the following pairs is wrongly
matched ?


354) Transgenic plants are the ones


355) The baeterium Bacillus thurmgiensis is
widely used in contemporary biology as


356) The genetically-modified (GM) brinjal in
India has beer developed for


357) Genetic engineering has been ijttjjfriiyrfefly
used for producing


358) Some of the characteristics of Bt cotton are


359) An improved variety of transgenic basmati
rice


360) Which one of the following is now being
commercially produced by biotechnological
procedures ?


361) The most common substrate used in
distilleries for the production of ethanol is


362) Maximum number of existing transgenic
animals is of


363) The process of RNA interference (RNAi)
has been used in the development of plants
resistant to


364) Continuous addition of sugars in ‘fed batch’
fermentation is done to


365) Bacillus thuringiensis forms protein crystals
which contain insecticidal protein. This
protein


366) Silencing of mRNA has been used in
producing transgenic plants resistant to


367) Read the following four statements (A-D)
about oenra irrmin in two of them
(A) The first transgenic buffalo, Rosie produced
milk was human alpha-lactalbumin enriched.
(B) Restriction enzymes are used in isolation of
DNA rrotr other macromolecules.
(C) Downstream processing is one of the steps
of rDNA technology.
(D) Disarmed pathogen vectors are also used in
transfer rDNA into the host.
Which of the two statements have mistakes?


368) Consumption of which one of the following
foods can prevent the kind of blindness
associated with vitamin A deficiency?


369) A patient brought to a hospital with myocardial infarction is normally
immediately -given


370) Tobacco plants resistant to a nematode have
been developed by the introduction of DNA
that produces (in the host cells)


371) The enzyme restriction endonuclease


372) Recombinant DNA technology is related
with


373) Hybridoma technology has been successfully
used in


374) Genetic engineering is


375) Which of the following is a recent application
of genetic engineering in diagnostic
technique?


376) Crown gall disease in plants is caused by


377) Polymerase chain reaction is most useful in


378) Most widely used bioweapon is


379) Golden rice is a transgenic crop of future
with following improved trait


380) Which enzyme is used as ‘molecular scissor’
in genetic engineering?


381) Protein-protein hybridisation results in


382) Which of the following is the product of
glucose fermented by yeast ?


383) Which enzyme helps in removing oil stains
from clothes


384) Which of the following enzymes is used in
genetic engineering ?


385) Which of the following is related to genetic
engineering ?


386) Known sequences of DNA that is used to
find complementary DNA strands is


387) Endonuclease is used in


388) Streptococcus is used for


389) Cheese is prepared by which enzyme ?


390) Abnormal gene is replaced by normal gene
through


391) Transgenic crop contains


392) Flavr Savr variety of tomato is a


393) The transgenic animals are those which have


394) Bt toxin is obtained from


395) Which of the following has been covered
under the patent category?


396) Which of the following organism is usid
m the pnxuca: human insulin by genetic
engineering?


397) Which of the following products is obtained
by genetic engineering ?


398) Electroporation involves


399) Which of the following is produced by
yeast?


400) Polymerase chain reaction is related with


401) Insect resistance transgenic cotton has been
produced by inserting a piece of DNA from


402) Which enzyme converts glucose into alcohol ?


403) Ethyl alcohol is obtained commercially on
a large scale from


404) Which one is used as vector in genetic
engineering ?


405) During “gene cloning” which is called “gene
taxi” ?


406) DNA probes are copied from the messenger
RNA molecules with the help of


407) Construction of a recombinant DNA
involves


408) Restriction endonucleases are most widely
used in recombinant DN A sechnologv. They
are obtained from


409) In Bt cotton, a transgenic plant, Bt refers to


410) Palaeontologists unearthed a human skull
during excavation. A small fragment of the
scalp tissue was still attached to it Only little
DNA could be extracted from it. If the genes
4 the ancient man need to be analysed, the
best way of sufficient amount of DNAfrom
this extract is


411) In genetic engineering recombinant DNA
means


412) Recognition sequence in Eco RI is


413) In genetic engineering experiment, DNA
fragments are joined by


414) Which is used in molecular genetic
engineering ?


415) Bacteria protect themselves from viruses by
fragmenting DNA upon entry with


416) The technique of production of monoclonal
antibodies developed by


417) Which of the following is maintained for
optimum productu of vinegar ?


418) In plant biotechnology, root tumours are
induced in plant usa the bacterium


419) The polymerase chain reaction is a technique
that


420) Monoclonal antibodies are produced from
which type cells ?


421) Gene therapy involve


422) The recent technique used for separating
DNA fragment is


423) Hybridomas are result of the talon of


424) Which one of the following combinations of
organisms are responsible for the formation
and flavour of yoghurt ?


425) First vitamin to be produced through
fermentation process using a wild bacterium
was


426) Gallic acid used in making ink is obtained
with the help of


427) The organism, used for alcohol fermentation,
is


428) Yeast (Saccharomyces cerevisiae) is used in
the industrial production of


429) Which of the following micro-organisms
is used for production of citric acid in
industries ?


430) The bacteria generally used for genetic
engineering is


431) First doped animal


432) Southern blot technique is used in


433) Dolly was created by


434) ANDI is genetically engineered


435) Eco RI is


436) Blunt ends of passenger and vehicle DNAs
are joined by


437) Bt toxin is


438) Insulin has 51 amino acids arranged in


439) Find the incorrect statement.


440) A bioreactor is


441) Genetically modified food can be Mtriiful
because it can Cause


442) Ti plasmid being used for introducing genes
in plants is obtained from


443) Who discovered recombinant DNA (rDNA)
technology


444) The technique in which foreign DNA is
precipitated over surface of metal particles
for passing into target cells is


445) Increased shelf life of tomato has been
achieved by


446) Which of these is used as vector in gene
therapy for SCID ?


447) The largest gene in man is


448) Recombinant DNA technology is also called


449) Restriction endonucleases are


450) Genetic engineering is possible because


451) The first transgenic crop was


452) A set of bacterial clones, each containing a
plasmid or phage, is called


453) Which of these are most widely used in
genetic engineering ?


454) Cultivation of Bt cotton has been much in
the news. The prefix Bt means


455) Which one of the following is a correct
statement ?


456) One method of biotechnology for Animal
Breeding Progranrna to improve yield of
milk/meat/wool in cattles, goat, sbesx. is
to incorporate new/foreign genes into the
genome of tber fertilized eggs to produce


457) Restriction enzymes present in several
micro-organism cm.foreign DNAatspecific sites and destroy them. The enzyraa do not
destroy the cellular DNA because


458) A transgenic plant is one into which


459) Transgenic plants are developed by


460) Plant cells can be converted to protoplasts
by treating them with


461) A vector for cloning genes in plants is


462) In recombinant DNA technology the term
vector refers to


463) ELISA stands for


464) The technique which involves addition or
deletion of genes is


465) Aspergillus niger produces


466) Which of the following isspecificlaly used
in genetic engineering ?


467) Plasmids are extrachromosomal circular
DNA molecules


468) One of the key factors, which makes the
plasmid a vector in genetic engineering is
that


469) Herbicide resistant gene in plants is


470) Transgenicjiants are


471) Which of the following DNA sequences
qualifies to be designated as a palindrome ?


472) The source of Taq polymerase used in PCR
is a


473) Cloning gene is a process where


474) Cloning does not provide


475) ‘Gray biotechnology’ referred to


476) Salt tolerant transgenic has been developed
for


477) Which of the following is produced by
genetically engineerec bacteria ?


478) Some of the steps involved in production
of humulin are given below. Choose the
correct sequence.
(i) Synthesis of gene (DNA) for human insulin
artificially.
(ii) Culturing recombinant E.coli in bioreactors.
(iii) Purification of hurrajjjirt.
(iv) Insertion of human ittSUlin gene into
plasmid.
(v) Introduction of recombinant plasmid into
E.coli.
(vi) Extraction of recombinant gene product
from E.coli.


479) GAATTC is the recognization site for which
of the following restriction endonuclease
?


480) Plants in comparison to animals are more
rapidly manipulated by genetic engineering.
Select out the niost probable reason for this


481) In recombinant DNA technique the term
vector refers to


482) The illegal told unlawful development
of biomaterials without payment to the
inhabitants of their origin is called


483) cry II Ab and cry I Ab produce toxins that
control


484) Which of the following is a plasmid ?


485) During the processing of the prohormone
“proinsulin”
into the mature “insulin”


486) The Bt toxin is not toxic to human beings
because


487) The thermostable enzymes, ‘Taq’and ‘Pfu’,
isolated from thermophilic bacteria are


488) If a recombinant DNA bearing gene for
ampicillin resistance is transferred into E.
coli cells and the host cells are spread on
agar plates containing ampicillin, then


489) The term,’Southern Blotting’ refers to


490) The purpose of polymerase chain reaction
is


491) Plasmids are


492) cDNA is


493) Biolistic technique is used in


494) Which of the following is correctly
matched ?


495) The technique of DNA fingerprinting was initially developed by


496) Silencing of a gene could be achieved by the use of


497) In callus culture, roots can be induced by the supply of


498) Golden rice
I. It is a transgenic variety of rice
II. It contains a goods quality of β-carotene (provitamin-A)
III. β-carotene is a principal source of vitamin-A
IV. The grains of the rice are yellow in colour due to β-carotene. The rice is commonly called golden rice Which of the statements given above are correct?


499) GEAC stands for


500) The linking of antibiotic resistance gene with the plasmid vector became possible with


501) Transgenic crops are modified through genetic engineering to develop natural resistance to insect pests. Which one is a transgenic plant?


502) Golden rice was created by transforming rice with two beta=carotene biosynthesis genes, namely,


503) Which of the following is used in genetic engineering?


504) Explants before organogenesis, is


505) In RNAi, genes are silenced using


506) GAATTC is the recognition site for the restriction endonuclease


507) Which gene was introduced in the first transgenic cow?


508) Which bacteria was the first to be used as biopesticide on the commercial scale in the world?


509) Silk is produced by


510) GM brinjal in India has been developed for resistance against


511) Which of the following is used as a best genetic vector in plants?


512) Somaclonal variation appears in plants


513) Which of the following cells cannot be grown under tissue which of culture condition?


514) Use of biological resources of other countries without any legal authorization of the countries concerned is called


515) The function of polymerase chain reaction is


516) Gene amplification using primers can be done by


517) A technique, which involves deliberate manipulation of genes within or between species is


518) Which of the following statement is not true for a clone?


519) Which of the following companies started selling humulin in 1983?


520) More advancement in genetic engineering is due to


521) An organisation aiming to regulate biotechnological activities was established by Indian Government named


522) Which of the following transgenic human protein product has been used to treat emphysema?


523) Biopiracy is


524) Meristem culture is practised in horticulture to get


525) Treatment of a genetic disorder by manipulating genes is called


526) PCR proceeds in three distinct steps governed by temperature, they are in order of


527) Lymphocytes are


528) Variable number of tandem repeats (VNTRs) in the DNA molecule are highly useful in


529) A clone is


530) In Bt cotton, transgenic plant, Bt refers to


531) The first time in 1990, M Blease and WF Andresco of National Institute of Health, attempted gene therapy on a 4 year old girl with which of the following enzyme deficiency?


532) Secondary cells can’t divide because


533) Undifferentiated mass of plant cells grown on nutrient medium, is called


534) The first case of IVF-ET technique success, was reported by


535) Today, transgenic models have been developed for many human diseases, which includes
I. rheumatoid arthritis II. Alzheimer’s disease
III. cancer
IV. Cystic fibrosis Choose the correct option


536) Probes, used in DNA fingerprinting, are initially


537) The decisions regarding the validity of GMO (Genetic Modification of Organism) research and the safety of introducing GM for the public services in India is taken by


538) Golden rice is


539) In order to obtain virus-free plants through tissue culture, the best method is


540) Which of the following techniques are related with gene therapy?
I. Bone marrow transplantation
II.Enzyme replacement therapy
III. Gel electrophoresis technique
IV. Hybridoma technique
Choose the correct option


541) The SCID patient has a defective gene for the enzyme Adenosine Deaminase (ADA). He/She lacks functional …… and therefore, fails to fight the infecting pathogens


542) The bacterium Bacillus thuringiensis is widely used in contemporary biology as a/an


543) The enzymes, commonly used in genetic engineering, are


544) In plant biotechnology, PEG is used in


545) Differentiation of shoot is controlled by


546) Which one of the following is used as vector for cloning genes into higher organisms?


547) Main objective of production/use of herbicide resistant GM crops is to


548) Golden rice is a transgenic crop of the future with which of the following improved trait?


549) Microbes found to be very useful in genetic engineering are


550) Molecular scissors are


551) Producing a giant mouse in the laboratory was possible through


552) Which of the following nematode infects the roots of the tobacco plants which reduce the production of tobacco?


553) ‘Roise’ cow known to produce a type of milk which has all the following characteristics
I. protein content of 2.4 g/L
II. human α-lactalbumin
III. more nutritionally balanced for human babies than natural cow milk Which of the above statements are correct?


554) The protein toxin producing bacteria, used to control biological pest, is


555) Which one of the following is correct explanation for autobiography?


556) A kind of biotechnology involving manipulation of DNA is


557) Which one of the following can help in the diagnosis of a genetical disorder?


558) A infects the roots of tobacco plants which reduce the production of tobacco


559) The term ‘totipotency’ refers to


560) Mixture of biogas contains


561) An efficient oil eating ‘Super bug’ developed through genetic engineering used in cleaning of oil-spill polluted sites is a species of


562) DNA fingerprinting refers to


563) A technology, which has found immense use in solving cases of disputed parentage, is


564) An extrachromosomal DNA, which can be used as vector in gene cloning is called


565) The protein products of the following Bt toxin genes cry I Ac and cry II Ab are responsible for controlling


566) Biopiracy is related to which of the following?


567) A suitable vector for gene cloning in higher organisms is


568) A correct pair of characteristic of molecular probe is
I. a single-stranded DNA or RNA tagged with a radioactive molecule
II. a double-stranded DNA tagged with a radioactive molecule
III. complementary to part of desired gene
IV. small molecule
Which of the above statements are correct?


569) In the initial stages of protoplast culture, sorbitol/mannitol is added


570) Important objectives of biotechnology in agriculture section are


571) Which of the following is/are considered as application (s) of biotechnology?
I. Waste treatment
II. Energy production
III. Bioremediation
IV. Processed food
V. Genetically modified crops for agriculture
VI. Diagnostics
VII. Therapeutics
Choose the correct option


572) The organism, which is used for gene transfer in higher organisms is


573) Which of the following statements are false?
I. Insulin for curing diabetes, used to be extracted from the pancreas of slaughtered pig and cattle
II. Animal insulin is slighty different from the human insulin
III. Animal insulin causes some undesirable side effects such as allergy
IV. Bacteria cannot be made to synthesise insulin from its gene because of the presence of introns Choose the correct option


574) Which of the following ways are suitable for increasing food production?
I. Agrochemical based agriculture
II. Organic agriculture
III. Genetically engineered crop-based agriculture Choose the correct option


575) Green revolution is related to the increase in production of


576) Tobacco plant resistant to a nematode have been developed by the introduction of DNA that produces (in the host cells)


577) Which one of the following pairs of term/names means one and the same thing?


578) At what temperature milk gets pasteurized?


579) In which of the following method, a probe is allowed to hybridise to its complementary DNA in the clone of cells?


580) Which of the following is/are correct about Adenosine Deaminase (ADA) deficiency?
I. In the absence of adenosine deaminase enzyme, purine metabolism is disturbed and T-lymphocytes fails to function
II. ADA deficiency is caused by the deletion of the gene for ADA
III. In some cases, it can be cured by bone marrow transplantation and enzyme replacement therapy. But in both approaches, the patients are not completely cured
IV. For permanent cure, genes isolated from the bone marrow cells producing ADA at early embryonic stages can be a possible cure
Which of the above statements are correct?


581) Which variety of rice was patented by a US company even through the highest number of varieties of this rice is found in India?


582) DNA fingerprinting technique was first developed by


583) DNA fingerprinting technique was first developed by


584) Both in callus and suspension cultures commonly used auxin is


585) A drug obtained through genetic engineering and useful for treating infertility is


586) According to NCERT text which Indian plants have either been patented or attempts have been made to patent them by Western nations for their commercial use?
I. Basmati rice
II. Neem
III. Turmic
IV. Tulsi


587) Plants, bacteria, fungi and animals whose genes have been altered by manipulation are called


588) Somaclonal variation is seen in


589) Which one of the following palindromic base sequences in DNA can be easily cut at about the middle by some particular restriction enzyme?


590) Crop plants grown in monoculture are


591) Agrochemical based agriculture includes


592) An improved variety of transgenic basmati rice


593) Plants are more rapidly manipulated by genetic engineering than animals due to


594) Test tube baby means, a baby born when


595) Genetically engineered bovine (bSI), sometimes called rbST (recombinant bovine somatotropin) or rbGH (recombinant bovine growth hormone) are used in the


596) Which one of the following is a correct statement?


597) Biopatents means


598) A USA patent was taken for


599) Fined the incorrect statement.


600) Some of the characteristics of Bt cotton are


601) Solution of polyethylene glycol (PEG) or a very brief high voltage electric current is used in fusion of


602) Transgenic animals are developed by


603) Correct chronological order of the steps occurring during gene therapy are
I. Lymphocytes are obtained from the patients
II. Lymphocytes are transferred to the culture dishes
III. Lymphocytes are transfected with the normal ADA genes
IV. The transfected cell are returned to the patients The chronological order should be


604) Manipulation of DNA in genetic engineering become easy due to invention of


605) Cry II Ab and cry I Ab produce toxins that control


606) Genetically engineered bacteria are being employed for production of


607) Micropropagation is a technique for production of


608) Which of the following radioisotope is not suitable for DNA labeling based studies?


609) Gene therapy in humans was first practiced by Blease and Andresco to cure


610) For production of haploids, we culture


611) Differentiation of organs and tissues in a developing organism, is associated with


612) How many varieties of rice has been estimated to be present in India?


613) A cybrid is hybrid carrying


614) Which of the following is correctly matched?


615) Which of the following shows correct chronological order of the events occurring during callus culture?


616) Which one of the following hydrolyses internal phosphodiester bonds in a polynucleotide chain?


617) White revolution is related to the increase in production


618) What is true about Bt toxin?


619) A plant species which has been exploited for the production of hirudin is


620) The aims and objectives of Genetic Engineering Approval Committee are
I. To permit the use of genetically modified organisms and their product for commercial applications
II. To adopt the procedures for restriction, production and application of GM organisms
III. approval to conduct large scale field trails and release of transgenic crops in the environment Which of the statements are given above are correct?


621) Which of the following is/are true?
I. Biowar is the use of biological weapons against humans and/or their crops and animals.
II. Bioethics is the unauthorized use of bioresources and traditional knowledge related to bioresources for commercial benefits.
III. Biopatent is exploitation of bioresources of other nations without proper authorization.


622) Alec Jeffreys developed the DNA fingerprinting technique. The probe he used was


623) ADA is an enzyme, which is found lacking in a genetic disorder SCID. What is the full form of ADA?


624) Cellular totipotency is demonstrated by


625) The problem of blindness in poor countries can be taken care of by using which of the following?


626) Consider the following statements about the responsibility of GEAC (set-up by the Indian Government)
I. GEAC make decisions regarding the validity of the GM research
II. It checks the safety of introducing GM organisms for the public services for their large scale use Which of the statements given above is/are correct?


627) All are the biotechnological application in order to increase food production, except


628) Which of the following is false for Bt transgenic plant?


629) DNA fingerprinting technique was discovered by


630) C-peptide of human insulin is


631) Consider the following statements about therapeutic drugs
I. The recombinant DNA technology is used for production of therapeutic drugs which are safe and effective
II. It avoid unwanted immunological responses, commonly observed with similar products isolated from non-human sources
III. About thirty recombinant therapeutics have been approved for human use in the world including India Which of the statements given above are correct?


632) Choose a correct option for the uses of PCR technique in diagnosis
I. It is used to detect HIV in suspected AIDS patients
II. It is used to detect mutations in the genes in suspected cancer patients
III. It is used to detect swine flu in human beings
IV. It is used to detect different common diseases in pigs, sheep and cow
V. It is a good technique to identify many other genetic disorders
Which of the above statements are correct?


633) What might be an advantage of beginning gene therapy prior to birth?


634) Which of the following transgenic animals are used in testing safety of polio vaccine before they are used on human?


635) Which Indian plants have either been patented or attempts have been made to patent them by Western nations for their use?


636) Which step was proved to be the main challenge in the production of human insulin by recombinant DNA technology?


637) A nutritionally wild type organism, which does not required any additional growth supplement is known as


638) The technique that was employed to produce haploids of Datura was


639) Find out the wrong statement.


640) Phytotron is


641) Which of the following bio-engineered bacteria is utilized for cleaning of marine oil slicks?


642) Which of the following peptide chain is removed during the maturation of proinsulin into insulin?


643) The enzyme employed for amplification of DNA during PCR is commercially obtained from


644) A genetically engineered microorganism used successfully in bioremediation of oil spills, is a species of


645) The vector for T-DNA is


646) What is true for plasmid?


647) β-carotene is a principal source of


648) Consider the following statements
I. Earlier, insulin was extracted from pancreas of slaughtered cattle and pigs which was more efficient than the genetically engineered insulin
II. PCR technique is being used for the detection of HIV in suspected AIDS patients and genetic mutations in suspected cancer patients
III. Cystic fibrosis, haemophilia, cancer, Parkinon’s etc., are treated by gene therapy Which of the statements given above are correct?


649) A single strand of nucleic acid tagged with a radioactive molecule is called


650) Product of biotechnology is


651) Enzyme that is used in PCR technology is


652) Transgenic animals are those which have foreign


653) The application of biotechnology includes all except


654) A strain of golden rice contains high contents of


655) Restriction endonucleases are enzymes which


656) Which one of the following is the most suitable, medium for culture of Drosophila melanogaster?


657) Technique used to detect the DNA in a clone is called


658) Genetic engineering is related with


659) The nucellar embryos were first produced by tissue culture technique in


660) Restriction endonucleases are


661) Kohler and Milstein developed a method in biotechnology for the production of


662) The haploid content of human DNA is


663) Human insulin is being commercially produced from a transgenic species of


664) The process of RNA interference has been used in the development of plants resistance to


665) Which one of the following techniques has helped to solve many mysteries involving murders, robberies and rapes?


666) Which one of the following bacterium is used for production of transgenic plants?


667) Agarose extracted from sea weeds finds use in


668) The inherent capacity of a cell to regenerate a new whole organism is called


669) Axenic culture is


670) Golden rice is a transgenic variety of rice which contains good quantities of


671) An institution, where valuable plant material likely to become irretrievably lost in the wild or in cultivation is preserved in viable condition is known as


672) Basmati is unique for its aroma and flavour, whose A… varieties are cultivated in B….Here A and B refers to


673) Which one of the following bacteria has found extensive use in genetic engineering work in plants?


674) Which one of the following gene is defective in patients suffering from Severe Combined Immunodeficiency Disease (SCID)?


675) Blood stains are found at the site of a murder. If DNA profiling technique is to be used for identifying the criminal, which of the following is ideal for use?


676) A probe which is a molecule used to locate specific sequences in a mixture of DNA or RNA molecules could be


677) Which of the following pairs are correctly matched?


678) Which one of the following is commonly used in transfer of foreign DNA into crop plants?


679) Production of value added products like nutrition supplements, pharmaceuticals, fuels, etc., using transgenic crop is called


680) Multicellular organisms that carries a specific genetic change in each cell because of an intervention at the fertilized egg stage is a


681) Polyethylene glycol method is used for


682) The site of the production of ADA in the body is


683) First genetically modified plants commercially released in India is


684) Which one of the following molecular diagnostic technique is used to detect the presence of a pathogen in its early stage of infection?


685) First hormone produced artificially by culture bacteria, is


686) In transgenics, expression of transgene in target tissue is determined by


687) Genomic DNA library means


688) The characteristics of a molecular probe are
I. Very long molecule
II. Double-stranded
III. DNA or RNA
IV. Complementary to a part of desired gene The correct pair is


689) A monopoly granted to a person who has either invented a new and useful article, made improvement in an existing article or invented a new process of making an article is called


690) Choose the correct option about agrochemicals


691) Some organisations and multinational companies patent biological resources of other nations without proper authorization from the countries concerned, this is called


692) Which one of the following is a ‘man made cereal’ not found in nature?


693) What is the demerit of using bovine insulin (from cow) and porcine insulin (from pig) in diabetic patients?


694) The tumour inducing capacity of Agrobacterium tumefaciens is located in large extra chromosomal plasmids called


695) Choose the correct options about Basmati rice
I. In 1997, an American company got patent rights for Basmati rice through the US Patent and Trademark office and was allowed to sell a ‘new variety’ in US and abroad
II. This new variety of Basmati was derived from Chinese farmer’s varieties
III. Indian Basmati was crossed with semidwarf varieties and claimed as an invention or a novelty Which of the above statements are correct?


696) Genetically engineered human insulin, humulin was launched by American drug company on


697) Which of the following is not a restriction endonuclease?


698) The totipotency of a cell refers to the


699) The anticoagulant hirudin is obtained from


700) Restriction enzymes are used to cut


701) When a person with defective Adenosine Deaminase (ADA) was treated, which of the following steps were performing for gene therapy?
I. Lymphocytes were extracted from the bone marrow of the patient
II. Lymphocytes were grown in a culture out side the body
III. Lymphocytes were transfected with the normal ADA genes
IV. The transfected cells were returned to the patients


702) DNA or RNA segment tagged with a radioactive molecule is called


703) There is a restriction endonuclease called Eco RI. What does ‘co’ part in it stand for?


704) Animals whose DNA is manipulated to possess and express an extra (foreign) gene are known as


705) Which of the following terms is used to describe the component isolated from a plant, for in vitro culturing in the specific medium?


706) Which of the following is a transgenic plant?


707) A doctor, while operating on an HIV+ patient accidently cut himself with a scalpel. He comes to you, suspecting himself to have contacted the virus. Which test will you advise him to rule out/confirm his suspicion?


708) Golden rice is a promising transgenic crop. When released for cultivation, it will help in


709) Natural genetic engineer is


710) Consider the following statements about ‘Rosie’
I. Rosie is a first transgenic cow
II. Rosie produced human protein enriched milk
III. The milk contained the human α-lactalbumin and scientist behind the research believes that the milk from the cow could provide an alternative to human breast milk
Which of the statements given above are correct?


711) An example of gene therapy is


712) The process, in which mature differentiated cells reverse to meristematic activity to form callus is called


713) The first human drug made by using genetic engineering technique was


714) In tissue culture, roots can be induced by


715) Consider the following statements about insulin
I. Human insulin is made-up of 51 amino acids arranged in two polypeptide chains
II. The two polypeptide chains are interconnected by two disulphide bridges
III. In mammals including humans, insulin is synthesized as a pro-hormone, which contains an extra stretch called the C-peptide
IV. C-peptide is not present in the mature insulin
Which of the statements given above are correct?


716) The basis of DNA fingerprinting is


717) Which of the following enzymes are used to join bits of DNA?


718) The construction of the first recombinant DNA was done by using the native plasmid of


719) Rules of conduct that may be used to regulate our activities in relation to the biological world is called


720) Murashige and Skoog’s medium is used for


721) Organic farming is the technique of raising crops by the use of


722) In crop improvement programmes, virus-free clones can be obtained through


723) _________ is a collection of methods that allows correction of gene defects diagnosed in a child or embryo


724) Consumption of which one of the following foods can prevent the kind of blindness associated with vitamin-A deficiency?


725) Which of the following statements are considered as the advantages of biotechnology?
I. Creation of fermented food
II. Production of pestresistant crops
III. Plants yielding more nutritious and tastier fruits
IV. Production of new types of medicine to fight dangerous disease
Choose the correct option


726) The name of first cloned sheep is


727) Palaeontologists unearthed a human skull during excavation. A small fragment of the scalp tissue was still attached to it. Only little DNA could be extracted from it. If the genes of the ancient man need to be analysed, the best way of getting sufficient amount of DNA from this extract is


728) Applications, like bioremediation, processed food, therapeutics and diagnostics are related with


729) The critical research areas of biotechnology are
I. providing best catalyst as improved organism, usually a microbes or pure enzyme
II. creating optimal conditions by engineering for a catalyst to act
III. down stream processing technologies
IV. Multiple Ovulation Transfer Technology (MOET)
Which of the statements given above the correct?


730) Part of the plant, which is cultured to obtain virus free clones, is


731) Which of the following is a plasmid?


732) Which of the following key factors, makes the plasmid, the vector in genetic engineering?


733) _________ animals are made to carry genes which make them more sensitive to the toxic substance than other more animals


734) Nucleic acid segment tagged with a radioactive molecule is called


735) Who discovered the super bug?


736) Automated DNA sequencers, work on the principle of the method developed by


737) Biotechnology mainly deals with


738) Restriction enzyme was discovered by


739) Which of the following techniques serves the purpose of early diagnosis of AIDS, cancer, etc?
I. Polymerase chain reaction
II. Recombinant DNA technology
III. Enzyme linked immune-sorbant assay
Choose the correct option


740) Adenosine Deaminase (ADA) deficiency can be cured by …A… and …B… but it is not fully curative. Here A and B can be


741) Which step has been taken by Government of India to cater to the requirements of patent terms and other emergency provisions in this regards


742) A regulatory body working under MoEF for the release of transgenic crops is


743) Gene therapy is


744) Which of the following could be a permanent cure for treatment of Severe Combined Immuno Deficiency (SCID)?


745) Hybridomas are result of the fusion of


746) The first clinical gene therapy was done for the treatment of


747) Transfer of any gene into a completely different organism can be done through


748) Somaclones are obtained by


749) Restriction endonucleases are most widely used in recombinant DNA technology. They are obtained from


750) Sterilization of tissue culture medium is done by


751) In cloning of cattle, a fertilized egg is taken out of the mother’s womb and


752) Which of these is used as vector in gene therapy for SCID?


753) About …A… recombinant therapeutics have been approved for human use the world over. In India, …B… of these are presently being marketed Here A and B can be


754) The mobile genetic element is


755) Choose the correct statement with reference to ‘Dolly’


756) The callus is not formed in


757) The green revolution succeeded in increasing the yield of crops mainly due to the use of
I. improved varieties of the crops
II. agro-chemicals
III. better management practices
Choose the correct option


758) Manipulation of DNA in genetic engineering became possible due to the discovery of


759) Which of the following is used in recombinant DNA technique?


760) Which one is regarded as a molecular scissor in biotechnology?


761) In 1997, the first transgenic cow, Rosie produced


762) Why a population fluctuate when it reaches to carrying capacity?


763) Small fish get stuck near the bottom of a shark and derives its nutrition from it. This kind of association is called as