Practice Test

Q1) Plant material used in tissue culture is Show Answer

Q2) When mature anthers of Datura inxonia are cultured in a culture medium supplemented with phytohormone named kinetin, coconut milk and plum juice, several embryos can be obtained floating inside the microsporangia. These embryos can develop into plants that are: Show Answer

Q3) Micropropagation is based on: Show Answer

Q4) The separation of single cell from the rest of the callus is: Show Answer

Q5) Konar and Nataraja demonstrated callus i.e., embryoids in buttercup also develops from: Show Answer

Q6) Any cell, tissue, or an organ removed from a plant for the purpose of culturing is called: Show Answer

Q7) An alkaloid which can be utilized to transform haploid plant into homozygous diploid plant is: Show Answer

Q8) The two enzymes utilized to isolate protoplasts from tissues or organs are: Show Answer

Q9) Fusion product of protoplast from the differect plants is termed: Show Answer

Q10) Potentiality of vegetative cells other than zygote to give rise to an independent plant is known as: Show Answer

Q11) First androgenic haploid was raised by: Show Answer

Q12) Microbes in the culture chamber are killed most suitably by: Show Answer

Q13) Non-pathological erratic growth of a plant tissue involving uncontrolled cell divisions is designated as: Show Answer

Q14) Triploid plants can be obtained from the culture of: Show Answer

Q15) Protoplast fusion or parasexual hybridization was first done between man and mouse cells by: Show Answer

Q16) Which of the following viruses have been extensively used to fuse cells in tissue culture: Show Answer

Q17) In gene therapy, DNA is inserted in a cell to compensate for: Show Answer

Q18) Eli Lilly and Co. is famous for: Show Answer

Q19) Callus and cancer both are undifferentiated mass of cells formed by uncontrolled mitosis. They differ as: Show Answer

Q20) Human growth hormone is now produced in large quantities by recombinant DNA technology. The previous source of this hormone, for treating pituitary dwarf, was: Show Answer

Q21) Plants that receive the foreign genes through genetic engineering are called: Show Answer

Q22) Which of the following correctly defines a transgenic animal: Show Answer

Q23) The first hormone artifically produced by culturing bacteria is: Show Answer

Q24) A somatic plant cell has potential to develop into a full plant. This is called: Show Answer

Q25) Abnormal gene is replaced by normal gene through: Show Answer

Q26) Gene therapy involves: Show Answer

Q27) Totipotency in cell is: Show Answer

Q28) An example of gene therapy is: Show Answer

Q29) Find the incorrect statement: Show Answer

Q30) An improved variety of transgenic basmati rice: Show Answer

Q31) Genetic engineering has been successfully used for producing: Show Answer

Q32) The genetically-modified (GM) brinjal in India has been developed for: Show Answer

Q33) Tissue culture technique has been biotechnologically successful in production of: Show Answer

Q34) The first relatively pure enzyme was prepared by: Show Answer

Q35) Yeast is a good source of: Show Answer

Q36) Germinating barley seeds are employed in the preparation of: Show Answer

Q37) Cells obtained from cancerous tumours are known as: Show Answer

Q38) As in Hepatitis B vaccine: Show Answer

Q39) Rennet enzyme was purified by: Show Answer

Q40) The enzyme diastase was identified by: Show Answer

Q41) Hybridomas are employed for: Show Answer

Q42) The method of growing microorganisms as a thin layer on nutrient medium is known as: Show Answer

Q43) Tissue plasminogen activator is: Show Answer

Q44) Dextran is used in: Show Answer

Q45) Which of these participates in the manufacturing of dextrans? Show Answer

Q46) Steroids are used in: Show Answer

Q47) Utility of fungi for steroid conversion was demonstrated by: Show Answer

Q48) The micro-organism involved in making bread is: Show Answer

Q49) Which of the following organic acids was produced by fermentation? Show Answer

Q50) Most of the Eubacteriale antibiotics come from: Show Answer

Q51) Hybridomas are a result of fusion of: Show Answer

Q52) Enzymes used in detergents are: Show Answer

Q53) Torulopsis utilis is: Show Answer

Q54) Antibiotics inhibit the growth of or destroy: Show Answer

Q55) If all the bacteria and fungi of the world are destroyed then: Show Answer

Q56) Streptomyces venezuelae is used to obtain: Show Answer

Q57) Citric acid is produced by: Show Answer

Q58) The first commercial example of enzyme modification of a protein for human use is: Show Answer

Q59) Which of the following is used for the diagnosis of disease caused by closely related pathogen? Show Answer

Q60) Utilization of living organisms/systems/processes for producing materials useful to human society is known as: Show Answer

Q61) Biotechnology utilizes new organisms developed from present organisms utilizing: Show Answer

Q62) Which of the following is not concerned with biotechnology? Show Answer

Q63) Which of these is used in biotechnology: Show Answer

Q64) Which of the following is used for manufacture of cosmetics? Show Answer

Q65) The technique by which virus detection can be make both in plants and animals is known as: Show Answer

Q66) Streptokinase (TPA) helps in: Show Answer

Q67) Interferons are: Show Answer

Q68) Cheese contains: Show Answer

Q69) Important objective of biotechnology in agriculture sector is: Show Answer

Q70) The main technique involved in agricultural biotechnology is called: Show Answer

Q71) The new strain of bacteria produced by biotechnology in alcohol industry is: Show Answer

Q72) Genetically engineered bacteria have been used in commercial production of: Show Answer

Q73) Which of the following correctly defines a transgenic animal? Show Answer

Q74) Which one of the following is not true about antibiotics? Show Answer

Q75) Rennin used in cheese industry is: Show Answer

Q76) The organism used for alcoholic fermentation is: Show Answer

Q77) A cell coded protein that is formed in response to infection with most animal viruses is called: Show Answer

Q78) Which of the following organelles is related with genetic engineering? Show Answer

Q79) A chemical susbtance derived from a living source that has the capacity to inhibit growth or to destroy microbes is called: Show Answer

Q80) The Central Drug Research Institute (CDRI) is situated in: Show Answer

Q81) Wine and Beer are produced directly by fermentation Brandy and Whisky require both fermentation and distillation because: Show Answer

Q82) The antibiotics obtained from Streptomyces are: Show Answer

Q83) During "gene cloning" which is called as "gene taxi"? Show Answer

Q84) What does Bt stands for the popular crop Bt cotton? Show Answer

Q85) Name the drug used in cancer treatment produced by using biotechnology: Show Answer

Q86) Use of biology in industrial process and for improving quality of life is called: Show Answer

Q87) What is the reason of formation of embryoid from pollen grain in tissue culture medium? Show Answer

Q88) The technique of obtaining large number of plantlets by tissue culture method is called: Show Answer

Q89) Somaclonal variation can be obtained by: Show Answer

Q90) The Ti plasmid is often used for making transgenic plants. This plasmid is found in: Show Answer

Q91) The first antibiotic was discovered by Show Answer

Q92) Colchicine brings about: Show Answer

Q93) Biogas produced by anaerobic decomposition used in combustion is mainly due to presence of: Show Answer

Q94) Which of the following is not an antibiotic? Show Answer

Q95) Which raw material is used in fermentation process of making beer? Show Answer

Q96) Which one of the following is the correctly matched pair of a product and the microorganism responsisble for it? Show Answer

Q97) India's wheat yield revolution in the 1960s was possible primarily due to: Show Answer

Q98) A self-fertilizing trihybrid plant forms: Show Answer

Q99) Bacillus thuringiensis (Bt) strains have been used for designing novel: Show Answer

Q100) Which of the following is generally used for induced mutagenesis in crop plants? Show Answer

Q101) Which antibiotic inhibits interaction between tRNA and mRNA during bacterial protein synthesis? Show Answer

Q102) Select the wrong statement: Show Answer

Q103) A transgenic food crop which may help in solving the problem of night blindness in developing countries is: Show Answer

Q104) Main objective of production/use of herbicide resistant GM crop is to: Show Answer

Q105) What is antisense technology? Show Answer

Q106) The process of RNA interference has been used in the development of plant resistant to: Show Answer

Q107) A plant part that is excised from its original location and used for initiating a culture is termed: Show Answer

Q108) Growth regulator commonly used in plant tissue culture is/are: Show Answer

Q109) Shoot regeneration is promoted by: Show Answer

Q110) Which of the following is used to obtain single cell protein used as food or feed? Show Answer

Q111) Protoplast of plant cell is obtained by working: Show Answer

Q112) Ringworm of scalp is caused by: Show Answer

Q113) An enzyme used in cheese making is: Show Answer

Q114) Biopiracy is related to which of the following? Show Answer

Q115) Which of the following is inculded in biopesticide? Show Answer

Q116) Which of the following can be controlled by using biopesticides? Show Answer

Q117) Which of the following risks are associated with genetically modified food?
I. Toxicity
II. Allergic reaction
III. Antibiotic resistance in microorganisms present in alimentary canal Show Answer

Q118) Embryo culture is used for: Show Answer

Q119) The plant cells without cell wall is called: Show Answer

Q120) Biotechnology deals with industrial scale production of biopharmaceuticals and biologicals using genetically modified Show Answer

Q121) Which of the following is for increasing food production? Show Answer

Q122) The crops having cry-genes need Show Answer

Q123) Cry-genes have been introduced in Show Answer

Q124) First transgenic crop is Show Answer

Q125) Bacillus thuringiensis is a bacterium of Show Answer

Q126) Which is not a transgenic plant? Show Answer

Q127) Bt toxin is Show Answer

Q128) Bt is resistant to Show Answer

Q129) Genetically modified tobacco plant with Bt gene is resistant to Show Answer

Q130) I. Recombinant DNA technology is used to improve crop plants by increasing their productivity, by making them more nutritious and by developing disease resistant.
II. Bt cotton is resistant to bollworm infestation.
III. Bacillus thuringiensis form cry protein during any phase of their growth.
IV. Bacillus thuringiensis is not harmed by self Cry protein because of its occurrence as protoxin (inactive).
V. Protoxin Cry protein is changed into active Cry protein in the stomach of insects due to alkaline pH in stomach. Show Answer

Q131) Bt gene produces protein toxin to insect larvae is Show Answer

Q132) A plant expressing a gene from another organisms is Show Answer

Q133) Introduction of transgene will result in Show Answer

Q134) Bt toxin is harmful to insects like Show Answer

Q135) How does Bt toxin kill the larvae of certain insects? Show Answer

Q136) Bt toxin genes are isolated from Bacillus thuringiensis and incorporated into crop plants making them insecticidal. The choice of genes depend upon Show Answer

Q137) Which of the following is correct? Show Answer

Q138) Bt cotton farming has shown good result in Show Answer

Q139) Several nematodes parasitise Show Answer

Q140) When DNA is transcribed into mRNA, usually the mRNA remains single-stranded, but in some cases an RNA can be made that is complementary to the mRNA. This is called _______ and its main function is to _______. Show Answer

Q141) Which of the following is the source of complementary strand in mRNA silencing Show Answer

Q142) Antisense RNA is Show Answer

Q143) Which of the following nematode infects the roots of tobacco plants and causes a great reduction in yield? Show Answer

Q144) The RNAi stands for Show Answer

Q145) mRNA silencing is called Show Answer

Q146) Fill up the blanks -
At present, about _________ recombinant therapeutics have been approved for human use the world over. In India ______ of these are presently being marketed. Show Answer

Q147) The first human drug made using recombinant DNA technology was Show Answer

Q148) Which of the following statements is false?
I. Insulin was originally extracted from pancreas of slaughtered pigs and cattle
II. Animal insulin is difficult to obtain
III. Animal insulin is identical to human insulin
IV. Non human insulin caused some patients to develop allergy
V. Recombinant DNA allowed scientists to insert a human insulin gene into a bacterial expression vector
VI. Recombinant insulin is actually obtained from E. coli bacterial cell Show Answer

Q149) RNA interference (RNAi) technique has been devised to protect the plants from nematode is silenced by ________ produced by the host plant. Show Answer

Q150) Eli Lilly, an American company prepared two DNA sequences corresponding to A and B, chains of human insulin and introduced them in plasmids of E. coli to produce insulin chains. Chains A and B were prepared separately, extracted and combined by creating Show Answer

Q151) Which step proved to be the main challenging obstacle in the production of human insulin by genetic engineering? Show Answer

Q152) Why is repeated transfusions of genetically engineered cells required in SCID patients? Show Answer

Q153) Human gene therapy requires Show Answer

Q154) When a patient with defective DNA was treated, which of the following steps was performed for gene therapy?
I. Lymphocytes were obtained from the patients
II. Lymphocytes are transferred to culture dishes
III. Lymphocytes were transfected with normal ADA genes
IV. The transfected cells are returned to the patients Show Answer

Q155) Which technique would you expect to be completely curative in SCID? Show Answer

Q156) Which of the following is a benefit to having insulin produced by biotechnology? Show Answer

Q157) Following are the steps in one type of gene therapy -
I. Inject engineered cells into patients bone marrow
II. Viral DNA carring the normal allele inserts into chromosome
III. Let retrovirus infect bone marrow cells that have removed from patient and cultured
IV. Insert RNA version of normal allele into retrovirus
Which of the following is correct sequence of steps Show Answer

Q158) Which of the following is correct about ADA? Show Answer

Q159) "Silencing" of mRNA molecule in order to control the production of a harmful protein has been used in protection of plants from Show Answer

Q160) For effective treatment of a disease Show Answer

Q161) Which of the following techniques serve the purpose of early diagnosis?
I. R-DNA technology
II. PCR
III. ELISA
IV. Convential method of diagnosis (serum, urine analysis etc.) Show Answer

Q162) A doctor while operating on an HIV + patient accidentally cut himself with a scalpel. He comes to you, suspecting himself to have contracted the virus which test will you advise him to rule out/confirm his suspicion? Show Answer

Q163) In order for gene therapy to be most effective, genes should be inserted in Show Answer

Q164) A nuclei acid probe might be used to Show Answer

Q165) Which of the following is a critically important tool used in experiments involving DNA hybridization? Show Answer

Q166) PCR is used to Show Answer

Q167) Which of the following is based upon the principle of antigen antibody interaction? Show Answer

Q168) DNA hybridization technique is based on all of the following properties of DNA except Show Answer

Q169) Which one of the following genes is defective in patients suffering from severe combined immuno-deficiency syndrome (SCID)? Show Answer

Q170) A functional ADA cDNA can be introduced into cells of the patients receiving gene therapy by using vector constituted by Show Answer

Q171) Deliberate alteration of genome for treatment of disease is called Show Answer

Q172) Which one is correct? Show Answer

Q173) Which of the following is correct? Show Answer

Q174) A correct pair of characteristics of molecular probe is -
A. Very long molecule
B. Double stranded
C. Single stranded DNA or RNA
D. Complementary to part of desired gene Show Answer

Q175) The DNA probe, 3' - GGCTTA, will hybridize with which of the following? Show Answer

Q176) Restriction enzymes were first discovered with observation that Show Answer

Q177) Following are the steps of southern blot procedure -
I. Autoradiography
II. Hybridization with radioactivity nucleic acid (probe)
III. Blotting
IV. DNA fragments are treated to make them single stranded
V. Electrophoresis
VI. Clearing of DNA of restriction endonuclease
VII. Isolation of DNA from sample
The correct sequence is Show Answer

Q178) Which method of cellular defence is common to all eukaryotic organisms? Show Answer

Q179) The technique by which "Dolly" the sheep was obtained is called Show Answer

Q180) Transgenic rats, rabbits, pigs, sheep, cows and fish have produced although over 95 percent of all existing transgenic animals are Show Answer

Q181) Transgenic animals are produced for which of the following purposes?
I. To study how gene are regulated and how they affect the normal functions of body and its development
II. To study of diseases
III. To obtain useful biological product
IV. To test vaccine safety and chemical safety Show Answer

Q182) In which disease the advancement of genetic engineering has still not been used as clinical cure Show Answer

Q183) "Rosie" a transgenic cow known to produce a type of milk which has all the following characteristics except Show Answer

Q184) Rosie was produced in year Show Answer

Q185) GMO/transgenic animal are used in testing safety of polio vaccine before they are used on human? Show Answer

Q186) To test the vaccine safety before its use on human, transgenic ______ are used to test. If successful, _______ are used to test the safety of vaccine Show Answer

Q187) Which of the following is correct? Show Answer

Q188) Which of the following statements is correct? Show Answer

Q189) Introduction of genetically modified food is not desirable because Show Answer

Q190) The use of bioresources by multinational companies and other organizations without proper authorization from the countries and people concerned without compensatory payment is called Show Answer

Q191) Which variety of rice was patented by a U.S. company even though the highest number of varieties of this rice is found in India? Show Answer

Q192) Which step of Government of India has taken to cater to the requirement of patent terms and other emergency provisions in this regard? Show Answer

Q193) A transgene expression can achieve which of the following? Show Answer

Q194) Which is being synthesized by genetic engineering? Show Answer

Q195) In some children, ADA deficiency can be cured by Show Answer

Q196) Bt toxin is produced by Show Answer

Q197) Today, transgenic models exist for many human diseases which includes -
A. Cancer
B. Cystic fibrosis
C. Rheumatoid arthritis
D. Alzhiemer's disease Show Answer

Q198) Main objective of producing herbicide resistant GM crops is Show Answer

Q199) Addition of foreign gene into a crop is Show Answer

Q200) Transgenic bacteria are being used for producing Show Answer

Q201) Transgenic Golden Rice is enriched with high Show Answer

Q202) A transgenic crop has genes for Show Answer

Q203) Reagent used in ELISA test is Show Answer

Q204) Which of the following cannot be patented Show Answer

Q205) Technique of production of monoclonal antibodies was developed by Show Answer

Q206) Genetic modification has -
A. made crops more tolerant to abiotic stresses.
B. reduced reliance on chemical pesticides.
C. increased efficiency of minerals use by plants.
D. enhanced nutritional value of food. Show Answer

Q207) Which of the following statement is not true. Show Answer

Q208) Which of the following statements is/are true about genetically engineered insulin?
A. its name is humulin
B. it was manufactured by American firm Eli Lilly
C. it was launched on 5 July 1983
D. it is produced by the fermentation of appropriate recombinant E. coli clones Show Answer

Q209) The actual process of splicing DNA fragments into plasmids takes place in Show Answer

Q210) Which Indian plants have either been patented or attempts have been made to patent them by western nations for their commercial uses.
A. Basmati rice
B. Turmeric
C. Neem
D. None Show Answer

Q211) The decisions regarding the validity of genetic modification research and the safety of introducing genetically modified organisms for public services in India is taken by Show Answer

Q212) The process of RNA interference takes place in all eukaryotic organisms as a method of cellular defense which -
A. Complementary dsRNA
B. Retroposons
C. Single Stranded DNA
D. Protein Show Answer

Q213) Which of following is/are correct?
A. gene therapy has been tested on a large number of patients with a wider variety of inherited genetic disorders, and in numerous cases it has produced a complete cure.
B. genetic engineering has been used to mass produce insuline for diabetes.
C. DNA hybridization is the base pairing of DNA from two different sources.
D. genetic engineering is a technique of plant breeding. Show Answer

Q214) Genetic engineering can be used to -
A. alter the performance of genetically modified organism.
B. produce multiple copies of a desired DNA sequence.
C. Generate many copies of specific genes.
D. Enhance the production of a specific gene product. Show Answer

Q215) Go through the statements.
A. During the processing of the prohormone "proinsulin" into the mature "insulin", C-peptide is removed from proinuslin.
B. In hydridoma technology, B-cells are fused with myeloma cells.
C. Genetic engineering has been successfully used for producing transgenic mice for testing safety of polio vaccin before use in humans.
D. Some of the characteristics of Bt cotton are high yield and resistance to bollworms.
E. An improved variety of transgenic basmati rice gives high yield and is rich in vitamin A.
F. 'Gray biotechnolgoy' is referred to industrial process.
G. The polymerase chain reaction is a technique that is used for in vivo replication of DNA.
How many statement is wrong? Show Answer

Q216) Which of the following is/are product(s) of genetic engineering -
A. Humulin
B. Hepatitis B vaccine prepared by yeast
C. Golden rice
D. Bt cotton Show Answer

Q217) Select the correct statement(s) Show Answer

Q218) Tobacco plants resistant to a nematode have been developed by the introduction of DNA that produced (in the host cells) Show Answer

Q219) Molecular probes used for identification of recombinant clone carrying the desired DNA insert can be -
A. denatured double stranded DNA probes
B. double stranded RNA probes
C. protein probes
D. single stranded DNA probes Show Answer

Q220) GM plants have been useful in increasing Show Answer

Q221) Proteins produced by Bacillus thuringiensis kill Show Answer

Q222) Which one is incorrect? Show Answer

Q223) Which is incorrect? Show Answer

Q224) What is correct about insulin? Show Answer

Q225) Genetically engineered human insulin is manufactured by the use of which of the following microorganisms? Show Answer

Q226) The first hormone produced artificially by culturing bacteria was Show Answer

Q227) Which enzyme is crucial for proper functioning of the immune system? Show Answer

Q228) Which statement is incorrect about gene therapy? Show Answer

Q229) PCR is routinely used to detect Show Answer

Q230) A genetically manipulated organism containing in its genome one or more inserted genes of another species is called Show Answer

Q231) It is now possible to breed plants and animals with desired characters through Show Answer

Q232) Use of bioresources of other nations without proper authorization of the countries concerned is called Show Answer

Q233) Which one is not correct? Show Answer

Q234) Bt cotton is resistant to Show Answer

Q235) Terminator gene Show Answer

Q236) Interferon is a glycoprotein which is Show Answer

Q237) Which statement is correct?
A. Somaclonal variations are the variations observed in plants regenerated from somatic cultures.
B. A transgenic crop is one that contains and expresses a transgene.
C. Chloromycetin is obtained from Penicillium.
D. Bioprospecting is the process of discovery and commercialization of new products based on biological resources. Show Answer

Q238) Which of the following is associated with transgenic animals?
A. Vaccine safety
B. Chemical safety
C. Biopiracy
D. Gene therapy Show Answer

Q239) Interferon is -
A. antimalarial
B. antifungal
C. antiviral
D. anticancer Show Answer

Q240) Humulin is -
A. an antibiotic
B. human insulin
C. antidiabetic
D. antifungal Show Answer

Q241) Which of the following is related with gene therapy?
A. Enzyme replacement therapy
B. Bone marrow transplantation
C. Hybridoma technique
D. Hepatitis Show Answer

Q242) The true statement about Genetic Engineering Approval committee (GEAC) is Show Answer

Q243) In hybridization Show Answer

Q244) DNA probes are used to Show Answer

Q245) For DNA probes to hybridize Show Answer

Q246) Statement - I : Non-homologous DNA will attach to a DNA probe.
Statement II : At high temperature, two complementary DNA strands will anneal. Show Answer

Q247) The term humulin is associated with Show Answer

Q248) Insect pest resistant Bt-cotton plant was developed using Show Answer

Q249) Find out the incorrect statement for transgenic animals Show Answer

Q250) Go through the following statements.
I. Human insulin is being commercially produced from a transgenic species of Escherichia coli.
II. The genetically modified Bacillus thuringiensis is used as biopesticide on the commercial scale.
III. Human protein, alpha-1-antitrypsin is used to treat emphysema.
IV. The first transgenic cow, Rosie produced alpha lactalbumin, enriched milk.
V. Bt toxin genes cry I Ac control the corn borer.
Select the wrong statement(s) Show Answer

Q251) The transgenic animals are generally produced for all the following needs except Show Answer

Q252) Which one of the following statements is wrong in relation to transgenic Bt cotton plant? Show Answer

Q253) Which body of the Government of Indian regulates GM research and safety of introducing GM organisms for public services? Show Answer

Q254) The crops engineered for glyphosate are resistant/tolerant to Show Answer

Q255) Golden rice is genetically modified crop plant where the incorporated gene is meant for biosynthesis of Show Answer

Q256) If any protein encoding gene is expressed in a heterologous host then protein is known as Show Answer

Q257) The two polypeptides of human insulin are linked together by Show Answer

Q258) Which part of the tobacco plant is infected by Meloidogyne incognita? Show Answer

Q259) Which kind of therapy was given in 1990 to a four year old girl with adenosine deaminase (ADA) deficiency? Show Answer

Q260) The first nif genes were isolated from Show Answer

Q261) ELISA is used to detect viruses where the
key reagent is Show Answer

Q262) Restriction endonucleases Show Answer

Q263) In transgenics, expression of transgene in
target tissue is determined by Show Answer

Q264) Gel electrophoresis is used for Show Answer

Q265) Polyethylene glycol method is used for Show Answer

Q266) Which one of the following is used as vector
for cloning into higher organisms? Show Answer

Q267) Restriction endonucleases are enzymes
which Show Answer

Q268) Stirred-tank bioreactors have been designed
for Show Answer

Q269) Which of the following are used in gene
cloning ? Show Answer

Q270) In genetic engineering, a DNA segment
(gene) of interexis transferred to the host
cell through a vector. Consider a following
four agents (i-iv) in this regard and select
the correct option about which one or more
of these can be used is vector/vectors
(i) Bacterium (ii) Plasmid
(iii) Plasmodium (iv) Bacteriophage Show Answer

Q271) Given below is a sample of a portion of
DNAstrand giving the base sequence on the
opposite strands. What is so specie shown
in it ?
5’___GAATTC____ 3’
3’____CTTAAG ____ 5’ Show Answer

Q272) There is a restriction endonuclease called
Eco R I. What does “co” part in it stand for? Show Answer

Q273) Which one of the following techniques made
it possible to genetically engineer living
organisms ? Show Answer

Q274) PCR and restriction fragment length
polymorphism are the methods for Show Answer

Q275) A singe strand of nucleic acid tagged with
a radioactive molecule is called Show Answer

Q276) Which one of the following is a case of
wrong matching ? Show Answer

Q277) Which one is a true statement regarding
DNA polymerase used in PCR ? Show Answer

Q278) For transformation, micro-particles coated
with DNA to be bombarded with gene gun
are made up of Show Answer

Q279) Biolistics (gene-gun) is suitable for Show Answer

Q280) In genetic engineering, the antibiotics are
used Show Answer

Q281) Which one of the following represents a
palindromic sequence in DNA ? Show Answer

Q282) Dough kept overnight in warm weather
becomes soft a spongy because of Show Answer

Q283) The Ti plasmid, is often used for making
transgenic roocs. The plasmid is found in Show Answer

Q284) Golden rice is a transgenic crop of the future
sac me improved trait Show Answer

Q285) Golden ride is a prortusing transgenic crop.
When released cultivation, it will help in Show Answer

Q286) Two microbes found to be very useful in
genetic engineering are Show Answer

Q287) What is antisense technology ? Show Answer

Q288) Cry I endotoxins obtained from Bacillus thuringiensis are effective against Show Answer

Q289) A transgenic food crap which may help in
solving the problem of night blindness in
developing countries is Show Answer

Q290) Main objective of production/use of
herbicide resistant crops is to Show Answer

Q291) Human insulin is being commercially ? Show Answer

Q292) Which one of the following is commonly
used in transfer of Foreign DNA into crop
plants? Show Answer

Q293) What is true about Bt toxin ? Show Answer

Q294) Which one of the following pairs is wrongly
matched ? Show Answer

Q295) Transgenic plants are the ones Show Answer

Q296) The baeterium Bacillus thurmgiensis is
widely used in contemporary biology as Show Answer

Q297) Some of the characteristics of Bt cotton are Show Answer

Q298) An improved variety of transgenic basmati
rice Show Answer

Q299) Which one of the following is now being
commercially produced by biotechnological
procedures ? Show Answer

Q300) The most common substrate used in
distilleries for the production of ethanol is Show Answer

Q301) Maximum number of existing transgenic
animals is of Show Answer

Q302) The process of RNA interference (RNAi)
has been used in the development of plants
resistant to Show Answer

Q303) Continuous addition of sugars in ‘fed batch’
fermentation is done to Show Answer

Q304) Bacillus thuringiensis forms protein crystals
which contain insecticidal protein. This
protein Show Answer

Q305) Silencing of mRNA has been used in
producing transgenic plants resistant to Show Answer

Q306) Read the following four statements (A-D)
about oenra irrmin in two of them
(A) The first transgenic buffalo, Rosie produced
milk was human alpha-lactalbumin enriched.
(B) Restriction enzymes are used in isolation of
DNA rrotr other macromolecules.
(C) Downstream processing is one of the steps
of rDNA technology.
(D) Disarmed pathogen vectors are also used in
transfer rDNA into the host.
Which of the two statements have mistakes? Show Answer

Q307) Consumption of which one of the following
foods can prevent the kind of blindness
associated with vitamin A deficiency? Show Answer

Q308) A patient brought to a hospital with myocardial infarction is normally
immediately -given Show Answer

Q309) Tobacco plants resistant to a nematode have
been developed by the introduction of DNA
that produces (in the host cells) Show Answer

Q310) The enzyme restriction endonuclease Show Answer

Q311) Recombinant DNA technology is related
with Show Answer

Q312) Hybridoma technology has been successfully
used in Show Answer

Q313) Which of the following is a recent application
of genetic engineering in diagnostic
technique? Show Answer

Q314) Crown gall disease in plants is caused by Show Answer

Q315) Polymerase chain reaction is most useful in Show Answer

Q316) Golden rice is a transgenic crop of future
with following improved trait Show Answer

Q317) Protein-protein hybridisation results in Show Answer

Q318) Which of the following is the product of
glucose fermented by yeast ? Show Answer

Q319) Which enzyme helps in removing oil stains
from clothes Show Answer

Q320) Known sequences of DNA that is used to
find complementary DNA strands is Show Answer

Q321) Streptococcus is used for Show Answer

Q322) Abnormal gene is replaced by normal gene
through Show Answer

Q323) Transgenic crop contains Show Answer

Q324) The transgenic animals are those which have Show Answer

Q325) Bt toxin is obtained from Show Answer

Q326) Which of the following products is obtained
by genetic engineering ? Show Answer

Q327) Insect resistance transgenic cotton has been
produced by inserting a piece of DNA from Show Answer

Q328) Which enzyme converts glucose into alcohol ? Show Answer

Q329) Ethyl alcohol is obtained commercially on
a large scale from Show Answer

Q330) DNA probes are copied from the messenger
RNA molecules with the help of Show Answer

Q331) Bacteria protect themselves from viruses by
fragmenting DNA upon entry with Show Answer

Q332) The technique of production of monoclonal
antibodies developed by Show Answer

Q333) Which of the following is maintained for
optimum production of vinegar ? Show Answer

Q334) The polymerase chain reaction is a technique
that Show Answer

Q335) Gene therapy involve Show Answer

Q336) The recent technique used for separating
DNA fragment is Show Answer

Q337) Which one of the following combinations of
organisms are responsible for the formation
and flavour of yoghurt ? Show Answer

Q338) First vitamin to be produced through
fermentation process using a wild bacterium
was Show Answer

Q339) Gallic acid used in making ink is obtained
with the help of Show Answer

Q340) The organism, used for alcohol fermentation,
is Show Answer

Q341) Yeast (Saccharomyces cerevisiae) is used in
the industrial production of Show Answer

Q342) Which of the following micro-organisms
is used for production of citric acid in
industries ? Show Answer

Q343) The bacteria generally used for genetic
engineering is Show Answer

Q344) Southern blot technique is used in Show Answer

Q345) ANDI is genetically engineered Show Answer

Q346) Eco RI is Show Answer

Q347) Blunt ends of passenger and vehicle DNAs
are joined by Show Answer

Q348) Bt toxin is Show Answer

Q349) Find the incorrect statement. Show Answer

Q350) A bioreactor is Show Answer

Q351) Ti plasmid being used for introducing genes
in plants is obtained from Show Answer

Q352) Who discovered recombinant DNA (rDNA)
technology Show Answer

Q353) The technique in which foreign DNA is
precipitated over surface of metal particles
for passing into target cells is Show Answer

Q354) Increased shelf life of tomato has been
achieved by Show Answer

Q355) The largest gene in man is Show Answer

Q356) Recombinant DNA technology is also called Show Answer

Q357) Restriction endonucleases are Show Answer

Q358) Genetic engineering is possible because Show Answer

Q359) The first transgenic crop was Show Answer

Q360) A set of bacterial clones, each containing a
plasmid or phage, is called Show Answer

Q361) Which of these are most widely used in
genetic engineering ? Show Answer

Q362) Cultivation of Bt cotton has been much in
the news. The prefix Bt means Show Answer

Q363) Which one of the following is a correct
statement ? Show Answer

Q364) One method of biotechnology for Animal
Breeding Progranrna to improve yield of
milk/meat/wool in cattles, goat, sbesx. is
to incorporate new/foreign genes into the
genome of tber fertilized eggs to produce Show Answer

Q365) Restriction enzymes present in several
micro-organism cm.foreign DNAatspecific sites and destroy them. The enzyraa do not
destroy the cellular DNA because Show Answer

Q366) A transgenic plant is one into which Show Answer

Q367) Transgenic plants are developed by Show Answer

Q368) Plant cells can be converted to protoplasts
by treating them with Show Answer

Q369) A vector for cloning genes in plants is Show Answer

Q370) In recombinant DNA technology the term
vector refers to Show Answer

Q371) ELISA stands for Show Answer

Q372) The technique which involves addition or
deletion of genes is Show Answer

Q373) Aspergillus niger produces Show Answer

Q374) Which of the following isspecificlaly used
in genetic engineering ? Show Answer

Q375) Plasmids are extrachromosomal circular
DNA molecules Show Answer

Q376) Herbicide resistant gene in plants is Show Answer

Q377) Transgenicjiants are Show Answer

Q378) Which of the following DNA sequences
qualifies to be designated as a palindrome ? Show Answer

Q379) The source of Taq polymerase used in PCR
is a Show Answer

Q380) Cloning gene is a process where Show Answer

Q381) Cloning does not provide Show Answer

Q382) Which of the following is produced by
genetically engineerec bacteria ? Show Answer

Q383) Some of the steps involved in production
of humulin are given below. Choose the
correct sequence.
(i) Synthesis of gene (DNA) for human insulin
artificially.
(ii) Culturing recombinant E.coli in bioreactors.
(iii) Purification of hurrajjjirt.
(iv) Insertion of human ittSUlin gene into
plasmid.
(v) Introduction of recombinant plasmid into
E.coli.
(vi) Extraction of recombinant gene product
from E.coli. Show Answer

Q384) GAATTC is the recognization site for which
of the following restriction endonuclease
? Show Answer

Q385) The illegal told unlawful development
of biomaterials without payment to the
inhabitants of their origin is called Show Answer

Q386) cry II Ab and cry I Ab produce toxins that
control Show Answer

Q387) Which of the following is a plasmid ? Show Answer

Q388) During the processing of the prohormone
“proinsulin”
into the mature “insulin” Show Answer

Q389) The Bt toxin is not toxic to human beings
because Show Answer

Q390) The thermostable enzymes, ‘Taq’and ‘Pfu’,
isolated from thermophilic bacteria are Show Answer

Q391) If a recombinant DNA bearing gene for
ampicillin resistance is transferred into E.
coli cells and the host cells are spread on
agar plates containing ampicillin, then Show Answer

Q392) cDNA is Show Answer

Q393) Which of the following is correctly
matched ? Show Answer

Q394) Which of the following in incorrectly matched? Show Answer

Q395) Cellular totipotency was first demonstrated by Show Answer

Q396) The technique of DNA fingerprinting was initially developed by Show Answer

Q397) Silencing of a gene could be achieved by the use of Show Answer

Q398) In callus culture, roots can be induced by the supply of Show Answer

Q399) Golden rice
I. It is a transgenic variety of rice
II. It contains a goods quality of β-carotene (provitamin-A)
III. β-carotene is a principal source of vitamin-A
IV. The grains of the rice are yellow in colour due to β-carotene. The rice is commonly called golden rice Which of the statements given above are correct? Show Answer

Q400) GEAC stands for Show Answer

Q401) Transgenic crops are modified through genetic engineering to develop natural resistance to insect pests. Which one is a transgenic plant? Show Answer

Q402) Golden rice was created by transforming rice with two beta=carotene biosynthesis genes, namely, Show Answer

Q403) In RNAi, genes are silenced using Show Answer

Q404) Which gene was introduced in the first transgenic cow? Show Answer

Q405) Which bacteria was the first to be used as biopesticide on the commercial scale in the world? Show Answer

Q406) Silk is produced by Show Answer

Q407) GM brinjal in India has been developed for resistance against Show Answer

Q408) Somaclonal variation appears in plants Show Answer

Q409) Which of the following cells cannot be grown under tissue which of culture condition? Show Answer

Q410) Use of biological resources of other countries without any legal authorization of the countries concerned is called Show Answer

Q411) Gene amplification using primers can be done by Show Answer

Q412) A technique, which involves deliberate manipulation of genes within or between species is Show Answer

Q413) Which of the following statement is not true for a clone? Show Answer

Q414) Which of the following companies started selling humulin in 1983? Show Answer

Q415) An organisation aiming to regulate biotechnological activities was established by Indian Government named Show Answer

Q416) Which of the following transgenic human protein product has been used to treat emphysema? Show Answer

Q417) Biopiracy is Show Answer

Q418) Meristem culture is practised in horticulture to get Show Answer

Q419) Treatment of a genetic disorder by manipulating genes is called Show Answer

Q420) PCR proceeds in three distinct steps governed by temperature, they are in order of Show Answer

Q421) Lymphocytes are Show Answer

Q422) Variable number of tandem repeats (VNTRs) in the DNA molecule are highly useful in Show Answer

Q423) A clone is Show Answer

Q424) In Bt cotton, transgenic plant, Bt refers to Show Answer

Q425) The first time in 1990, M Blease and WF Andresco of National Institute of Health, attempted gene therapy on a 4 year old girl with which of the following enzyme deficiency? Show Answer

Q426) Secondary cells can’t divide because Show Answer

Q427) Undifferentiated mass of plant cells grown on nutrient medium, is called Show Answer

Q428) The first case of IVF-ET technique success, was reported by Show Answer

Q429) Today, transgenic models have been developed for many human diseases, which includes
I. rheumatoid arthritis II. Alzheimer’s disease
III. cancer
IV. Cystic fibrosis Choose the correct option Show Answer

Q430) Probes, used in DNA fingerprinting, are initially Show Answer

Q431) The decisions regarding the validity of GMO (Genetic Modification of Organism) research and the safety of introducing GM for the public services in India is taken by Show Answer

Q432) Golden rice is Show Answer

Q433) In order to obtain virus-free plants through tissue culture, the best method is Show Answer

Q434) Which of the following techniques are related with gene therapy?
I. Bone marrow transplantation
II.Enzyme replacement therapy
III. Gel electrophoresis technique
IV. Hybridoma technique
Choose the correct option Show Answer

Q435) The SCID patient has a defective gene for the enzyme Adenosine Deaminase (ADA). He/She lacks functional …… and therefore, fails to fight the infecting pathogens Show Answer

Q436) The bacterium Bacillus thuringiensis is widely used in contemporary biology as a/an Show Answer

Q437) The enzymes, commonly used in genetic engineering, are Show Answer

Q438) In plant biotechnology, PEG is used in Show Answer

Q439) Differentiation of shoot is controlled by Show Answer

Q440) Which one of the following is used as vector for cloning genes into higher organisms? Show Answer

Q441) Main objective of production/use of herbicide resistant GM crops is to Show Answer

Q442) Golden rice is a transgenic crop of the future with which of the following improved trait? Show Answer

Q443) Microbes found to be very useful in genetic engineering are Show Answer

Q444) Molecular scissors are Show Answer

Q445) Producing a giant mouse in the laboratory was possible through Show Answer

Q446) Which of the following nematode infects the roots of the tobacco plants which reduce the production of tobacco? Show Answer

Q447) ‘Roise’ cow known to produce a type of milk which has all the following characteristics
I. protein content of 2.4 g/L
II. human α-lactalbumin
III. more nutritionally balanced for human babies than natural cow milk Which of the above statements are correct? Show Answer

Q448) The protein toxin producing bacteria, used to control biological pest, is Show Answer

Q449) Which one of the following is correct explanation for autobiography? Show Answer

Q450) A kind of biotechnology involving manipulation of DNA is Show Answer

Q451) Which one of the following can help in the diagnosis of a genetical disorder? Show Answer

Q452) A infects the roots of tobacco plants which reduce the production of tobacco Show Answer

Q453) The term ‘totipotency’ refers to Show Answer

Q454) Mixture of biogas contains Show Answer

Q455) DNA fingerprinting refers to Show Answer

Q456) An extrachromosomal DNA, which can be used as vector in gene cloning is called Show Answer

Q457) The protein products of the following Bt toxin genes cry I Ac and cry II Ab are responsible for controlling Show Answer

Q458) A suitable vector for gene cloning in higher organisms is Show Answer

Q459) A correct pair of characteristic of molecular probe is
I. a single-stranded DNA or RNA tagged with a radioactive molecule
II. a double-stranded DNA tagged with a radioactive molecule
III. complementary to part of desired gene
IV. small molecule
Which of the above statements are correct? Show Answer

Q460) In the initial stages of protoplast culture, sorbitol/mannitol is added Show Answer

Q461) Important objectives of biotechnology in agriculture section are Show Answer

Q462) Which of the following is/are considered as application (s) of biotechnology?
I. Waste treatment
II. Energy production
III. Bioremediation
IV. Processed food
V. Genetically modified crops for agriculture
VI. Diagnostics
VII. Therapeutics
Choose the correct option Show Answer

Q463) The organism, which is used for gene transfer in higher organisms is Show Answer

Q464) Which of the following statements are false?
I. Insulin for curing diabetes, used to be extracted from the pancreas of slaughtered pig and cattle
II. Animal insulin is slighty different from the human insulin
III. Animal insulin causes some undesirable side effects such as allergy
IV. Bacteria cannot be made to synthesise insulin from its gene because of the presence of introns Choose the correct option Show Answer

Q465) Which of the following ways are suitable for increasing food production?
I. Agrochemical based agriculture
II. Organic agriculture
III. Genetically engineered crop-based agriculture Choose the correct option Show Answer

Q466) Tobacco plant resistant to a nematode have been developed by the introduction of DNA that produces (in the host cells) Show Answer

Q467) Which one of the following pairs of term/names means one and the same thing?
Show Answer

Q468) In which of the following method, a probe is allowed to hybridise to its complementary DNA in the clone of cells? Show Answer

Q469) Which of the following is/are correct about Adenosine Deaminase (ADA) deficiency?
I. In the absence of adenosine deaminase enzyme, purine metabolism is disturbed and T-lymphocytes fails to function
II. ADA deficiency is caused by the deletion of the gene for ADA
III. In some cases, it can be cured by bone marrow transplantation and enzyme replacement therapy. But in both approaches, the patients are not completely cured
IV. For permanent cure, genes isolated from the bone marrow cells producing ADA at early embryonic stages can be a possible cure
Which of the above statements are correct? Show Answer

Q470) Which variety of rice was patented by a US company even through the highest number of varieties of this rice is found in India? Show Answer

Q471) Both in callus and suspension cultures commonly used auxin is Show Answer

Q472) A drug obtained through genetic engineering and useful for treating infertility is Show Answer

Q473) According to NCERT text which Indian plants have either been patented or attempts have been made to patent them by Western nations for their commercial use?
I. Basmati rice
II. Neem
III. Turmic
IV. Tulsi
Show Answer

Q474) Plants, bacteria, fungi and animals whose genes have been altered by manipulation are called
Show Answer

Q475) Somaclonal variation is seen in Show Answer

Q476) Which one of the following palindromic base sequences in DNA can be easily cut at about the middle by some particular restriction enzyme? Show Answer

Q477) Crop plants grown in monoculture are Show Answer

Q478) Agrochemical based agriculture includes Show Answer

Q479) An improved variety of transgenic basmati rice Show Answer

Q480) Plants are more rapidly manipulated by genetic engineering than animals due to Show Answer

Q481) Test tube baby means, a baby born when Show Answer

Q482) Genetically engineered bovine (bSI), sometimes called rbST (recombinant bovine somatotropin) or rbGH (recombinant bovine growth hormone) are used in the Show Answer

Q483) Biopatents means Show Answer

Q484) A USA patent was taken for Show Answer

Q485) Solution of polyethylene glycol (PEG) or a very brief high voltage electric current is used in fusion of Show Answer

Q486) Transgenic animals are developed by Show Answer

Q487) Correct chronological order of the steps occurring during gene therapy are
I. Lymphocytes are obtained from the patients
II. Lymphocytes are transferred to the culture dishes
III. Lymphocytes are transfected with the normal ADA genes
IV. The transfected cell are returned to the patients The chronological order should be Show Answer

Q488) Cry II Ab and cry I Ab produce toxins that control Show Answer

Q489) Genetically engineered bacteria are being employed for production of Show Answer

Q490) Micropropagation is a technique for production of Show Answer

Q491) Which of the following radioisotope is not suitable for DNA labeling based studies? Show Answer

Q492) Gene therapy in humans was first practiced by Blease and Andresco to cure Show Answer

Q493) For production of haploids, we culture Show Answer

Q494) Differentiation of organs and tissues in a developing organism, is associated with Show Answer

Q495) How many varieties of rice has been estimated to be present in India? Show Answer

Q496) Which of the following shows correct chronological order of the events occurring during callus culture? Show Answer

Q497) Which one of the following hydrolyses internal phosphodiester bonds in a polynucleotide chain? Show Answer

Q498) White revolution is related to the increase in production Show Answer

Q499) What is true about Bt toxin? Show Answer

Q500) The aims and objectives of Genetic Engineering Approval Committee are
I. To permit the use of genetically modified organisms and their product for commercial applications
II. To adopt the procedures for restriction, production and application of GM organisms
III. approval to conduct large scale field trails and release of transgenic crops in the environment Which of the statements are given above are correct? Show Answer

Q501) Alec Jeffreys developed the DNA fingerprinting technique. The probe he used was Show Answer

Q502) ADA is an enzyme, which is found lacking in a genetic disorder SCID. What is the full form of ADA? Show Answer

Q503) Cellular totipotency is demonstrated by Show Answer

Q504) The problem of blindness in poor countries can be taken care of by using which of the following? Show Answer

Q505) Consider the following statements about the responsibility of GEAC (set-up by the Indian Government)
I. GEAC make decisions regarding the validity of the GM research
II. It checks the safety of introducing GM organisms for the public services for their large scale use Which of the statements given above is/are correct? Show Answer

Q506) All are the biotechnological application in order to increase food production, except Show Answer

Q507) Which of the following is false for Bt transgenic plant? Show Answer

Q508) C-peptide of human insulin is Show Answer

Q509) Consider the following statements about therapeutic drugs
I. The recombinant DNA technology is used for production of therapeutic drugs which are safe and effective
II. It avoid unwanted immunological responses, commonly observed with similar products isolated from non-human sources
III. About thirty recombinant therapeutics have been approved for human use in the world including India Which of the statements given above are correct? Show Answer

Q510) Choose a correct option for the uses of PCR technique in diagnosis
I. It is used to detect HIV in suspected AIDS patients
II. It is used to detect mutations in the genes in suspected cancer patients
III. It is used to detect swine flu in human beings
IV. It is used to detect different common diseases in pigs, sheep and cow
V. It is a good technique to identify many other genetic disorders
Which of the above statements are correct? Show Answer

Q511) What might be an advantage of beginning gene therapy prior to birth? Show Answer

Q512) Which of the following transgenic animals are used in testing safety of polio vaccine before they are used on human? Show Answer

Q513) Which Indian plants have either been patented or attempts have been made to patent them by Western nations for their use? Show Answer

Q514) Which step was proved to be the main challenge in the production of human insulin by recombinant DNA technology? Show Answer

Q515) A nutritionally wild type organism, which does not required any additional growth supplement is known as Show Answer

Q516) The technique that was employed to produce haploids of Datura was Show Answer

Q517) Find out the wrong statement.
Show Answer

Q518) Which of the following bio-engineered bacteria is utilized for cleaning of marine oil slicks? Show Answer

Q519) Which of the following peptide chain is removed during the maturation of proinsulin into insulin? Show Answer

Q520) A genetically engineered microorganism used successfully in bioremediation of oil spills, is a species of Show Answer

Q521) The vector for T-DNA is Show Answer

Q522) What is true for plasmid? Show Answer

Q523) β-carotene is a principal source of Show Answer

Q524) Consider the following statements
I. Earlier, insulin was extracted from pancreas of slaughtered cattle and pigs which was more efficient than the genetically engineered insulin
II. PCR technique is being used for the detection of HIV in suspected AIDS patients and genetic mutations in suspected cancer patients
III. Cystic fibrosis, haemophilia, cancer, Parkinon’s etc., are treated by gene therapy Which of the statements given above are correct? Show Answer

Q525) Product of biotechnology is Show Answer

Q526) Enzyme that is used in PCR technology is Show Answer

Q527) The application of biotechnology includes all except Show Answer

Q528) A strain of golden rice contains high contents of Show Answer

Q529) Which one of the following is the most suitable, medium for culture of Drosophila melanogaster? Show Answer

Q530) Technique used to detect the DNA in a clone is called Show Answer

Q531) Genetic engineering is related with Show Answer

Q532) The nucellar embryos were first produced by tissue culture technique in Show Answer

Q533) The haploid content of human DNA is Show Answer

Q534) Human insulin is being commercially produced from a transgenic species of Show Answer

Q535) Which one of the following techniques has helped to solve many mysteries involving murders, robberies and rapes? Show Answer

Q536) Which one of the following bacterium is used for production of transgenic plants? Show Answer

Q537) Agarose extracted from sea weeds finds use in Show Answer

Q538) The inherent capacity of a cell to regenerate a new whole organism is called Show Answer

Q539) Axenic culture is Show Answer

Q540) Golden rice is a transgenic variety of rice which contains good quantities of Show Answer

Q541) An institution, where valuable plant material likely to become irretrievably lost in the wild or in cultivation is preserved in viable condition is known as Show Answer

Q542) Which one of the following bacteria has found extensive use in genetic engineering work in plants? Show Answer

Q543) Which one of the following gene is defective in patients suffering from Severe Combined Immunodeficiency Disease (SCID)? Show Answer

Q544) Blood stains are found at the site of a murder. If DNA profiling technique is to be used for identifying the criminal, which of the following is ideal for use? Show Answer

Q545) A probe which is a molecule used to locate specific sequences in a mixture of DNA or RNA molecules could be Show Answer

Q546) Which of the following pairs are correctly matched? Show Answer

Q547) Which one of the following is commonly used in transfer of foreign DNA into crop plants? Show Answer

Q548) Production of value added products like nutrition supplements, pharmaceuticals, fuels, etc., using transgenic crop is called Show Answer

Q549) Multicellular organisms that carries a specific genetic change in each cell because of an intervention at the fertilized egg stage is a Show Answer

Q550) The site of the production of ADA in the body is Show Answer

Q551) First genetically modified plants commercially released in India is Show Answer

Q552) Which one of the following molecular diagnostic technique is used to detect the presence of a pathogen in its early stage of infection? Show Answer

Q553) First hormone produced artificially by culture bacteria, is Show Answer

Q554) In transgenics, expression of transgene in target tissue is determined by Show Answer

Q555) Genomic DNA library means Show Answer

Q556) The characteristics of a molecular probe are
I. Very long molecule
II. Double-stranded
III. DNA or RNA
IV. Complementary to a part of desired gene The correct pair is Show Answer

Q557) A monopoly granted to a person who has either invented a new and useful article, made improvement in an existing article or invented a new process of making an article is called Show Answer

Q558) Choose the correct option about agrochemicals Show Answer

Q559) Some organisations and multinational companies patent biological resources of other nations without proper authorization from the countries concerned, this is called Show Answer

Q560) Which one of the following is a ‘man made cereal’ not found in nature? Show Answer

Q561) What is the demerit of using bovine insulin (from cow) and porcine insulin (from pig) in diabetic patients? Show Answer

Q562) The tumour inducing capacity of Agrobacterium tumefaciens is located in large extra chromosomal plasmids called Show Answer

Q563) Choose the correct options about Basmati rice
I. In 1997, an American company got patent rights for Basmati rice through the US Patent and Trademark office and was allowed to sell a ‘new variety’ in US and abroad
II. This new variety of Basmati was derived from Chinese farmer’s varieties
III. Indian Basmati was crossed with semidwarf varieties and claimed as an invention or a novelty Which of the above statements are correct? Show Answer

Q564) Which of the following is not a restriction endonuclease? Show Answer

Q565) The totipotency of a cell refers to the Show Answer

Q566) The anticoagulant hirudin is obtained from Show Answer

Q567) Restriction enzymes are used to cut Show Answer

Q568) When a person with defective Adenosine Deaminase (ADA) was treated, which of the following steps were performing for gene therapy?
I. Lymphocytes were extracted from the bone marrow of the patient
II. Lymphocytes were grown in a culture out side the body
III. Lymphocytes were transfected with the normal ADA genes
IV. The transfected cells were returned to the patients Show Answer

Q569) There is a restriction endonuclease called Eco RI. What does ‘co’ part in it stand for? Show Answer

Q570) Animals whose DNA is manipulated to possess and express an extra (foreign) gene are known as Show Answer

Q571) Which of the following terms is used to describe the component isolated from a plant, for in vitro culturing in the specific medium? Show Answer

Q572) Which of the following is a transgenic plant? Show Answer

Q573) A doctor, while operating on an HIV+ patient accidently cut himself with a scalpel. He comes to you, suspecting himself to have contacted the virus. Which test will you advise him to rule out/confirm his suspicion? Show Answer

Q574) Golden rice is a promising transgenic crop. When released for cultivation, it will help in Show Answer

Q575) Natural genetic engineer is Show Answer

Q576) Consider the following statements about ‘Rosie’
I. Rosie is a first transgenic cow
II. Rosie produced human protein enriched milk
III. The milk contained the human α-lactalbumin and scientist behind the research believes that the milk from the cow could provide an alternative to human breast milk
Which of the statements given above are correct? Show Answer

Q577) An example of gene therapy is Show Answer

Q578) The process, in which mature differentiated cells reverse to meristematic activity to form callus is called Show Answer

Q579) The first human drug made by using genetic engineering technique was Show Answer

Q580) Consider the following statements about insulin
I. Human insulin is made-up of 51 amino acids arranged in two polypeptide chains
II. The two polypeptide chains are interconnected by two disulphide bridges
III. In mammals including humans, insulin is synthesized as a pro-hormone, which contains an extra stretch called the C-peptide
IV. C-peptide is not present in the mature insulin
Which of the statements given above are correct? Show Answer

Q581) The basis of DNA fingerprinting is Show Answer

Q582) Which of the following enzymes are used to join bits of DNA? Show Answer

Q583) The construction of the first recombinant DNA was done by using the native plasmid of Show Answer

Q584) Rules of conduct that may be used to regulate our activities in relation to the biological world is called Show Answer

Q585) Murashige and Skoog’s medium is used for Show Answer

Q586) Organic farming is the technique of raising crops by the use of Show Answer

Q587) In crop improvement programmes, virus-free clones can be obtained through Show Answer

Q588) _________ is a collection of methods that allows correction of gene defects diagnosed in a child or embryo Show Answer

Q589) Consumption of which one of the following foods can prevent the kind of blindness associated with vitamin-A deficiency? Show Answer

Q590) Which of the following statements are considered as the advantages of biotechnology?
I. Creation of fermented food
II. Production of pestresistant crops
III. Plants yielding more nutritious and tastier fruits
IV. Production of new types of medicine to fight dangerous disease
Choose the correct option Show Answer

Q591) Applications, like bioremediation, processed food, therapeutics and diagnostics are related with Show Answer

Q592) The critical research areas of biotechnology are
I. providing best catalyst as improved organism, usually a microbes or pure enzyme
II. creating optimal conditions by engineering for a catalyst to act
III. down stream processing technologies
IV. Multiple Ovulation Transfer Technology (MOET)
Which of the statements given above the correct? Show Answer

Q593) Part of the plant, which is cultured to obtain virus free clones, is Show Answer

Q594) _________ animals are made to carry genes which make them more sensitive to the toxic substance than other more animals Show Answer

Q595) Biotechnology mainly deals with Show Answer

Q596) Which of the following techniques serves the purpose of early diagnosis of AIDS, cancer, etc?
I. Polymerase chain reaction
II. Recombinant DNA technology
III. Enzyme linked immune-sorbant assay
Choose the correct option Show Answer

Q597) Adenosine Deaminase (ADA) deficiency can be cured by …A… and …B… but it is not fully curative. Here A and B can be Show Answer

Q598) Which step has been taken by Government of India to cater to the requirements of patent terms and other emergency provisions in this regards Show Answer

Q599) Gene therapy is Show Answer

Q600) Which of the following could be a permanent cure for treatment of Severe Combined Immuno Deficiency (SCID)? Show Answer

Q601) Hybridomas are result of the fusion of Show Answer

Q602) The first clinical gene therapy was done for the treatment of Show Answer

Q603) Transfer of any gene into a completely different organism can be done through Show Answer

Q604) Somaclones are obtained by Show Answer

Q605) In cloning of cattle, a fertilized egg is taken out of the mother’s womb and Show Answer

Q606) Which of these is used as vector in gene therapy for SCID? Show Answer

Q607) The mobile genetic element is Show Answer

Q608) The callus is not formed in Show Answer

Q609) In 1997, the first transgenic cow, Rosie produced Show Answer

Q610) An example of gene therapy is Show Answer

Q611) Protoplasts of two different species are used in Show Answer

Q612) Which of the following genetically engineered. organisms was used by Eli Lilly to prepare human insulin?
Show Answer

Q613) Silencing of specific mRNA is possible via RNAi because of -
Show Answer

Q614) Why can't insulin be given orally to diabetic patients?
Show Answer

Q615) Which of the following enzyme(s) are NOT essential for gene cloning?
A. Restriction enzymes
B. DNA ligase
C. DNA mutase
D. DNA recombinase
E. DNA polymerase
Choose the correct answer from the options given below:
Show Answer

Q616) The capacity to generate a whole plant from any cell of the plant is
called: Show Answer

Q617) Given below are two statements:
Statement I : Bt toxins are insect group specific and coded by a gene cry
IAc.
Statement II : Bt toxin exists as inactive protoxin in B. thuringiensis.
However, after ingestion by the insect the inactive protoxin gets
converted into active form due to acidic pH of the insect gut.
In the light of the above statements, choose the correct answer from the
options given below: Show Answer

Q618) You are given a tissue with its potential for differentiation in an
artificial culture. Which of the following pairs of hormones would you
add to the medium to secure shoots as well as roots? Show Answer

Q619) Following are the steps involved in action of toxin in Bt. Cotton
A. The inactive toxin converted into active form due to alkaline pH of
gut of insect.
B. Bacillus thuringiensis produce crystals with toxic insecticidal
proteins.
C. The alkaline pH solubilises the crystals.
D. The activated toxin binds to the surface of midgut cells, creates pores
and causes death of the insect.
E. The toxin proteins exist as inactive protoxins in bacteria.
Choose the correct sequence of steps from the options given below: Show Answer

Q620) What is the fate of a piece of DNA carrying only gene of interest which
is transferred into an alien organism?
A. The piece of DNA would be able to multiply itself independently in
the progeny cells of the organism.
B. It may get integrated into the genome of the recipient.
C. It may multiply and be inherited along with the host DNA.
D. The alien piece of DNA is not an integral part of chromosome.
E. It shows ability to replicate.
Choose the correct answer from the options given below: Show Answer

Q621) Which of the following are fused in somatic hybridization involving two
varieties of plants? Show Answer

Q622) Which of the following statements is incorrect? Show Answer

Q623) Which of the following statement is incorrect about Agrobacterium
tumifaciens? Show Answer

Q624) Which of the following can act as molecular scissors? Show Answer

Q625) Which one of the following techniques does not serve the purpose of
early diagnosis of a disease for its early treatment? Show Answer

Q626) Milk of transgenic 'Cow Rosie' was nutritionally more balanced product
for human babies than natural cow milk because it contained : Show Answer

Q627) Transposons can be used during which one of the following ? Show Answer

Q628) In gene therapy of Adenosine Deaminase (ADA) deficiency, the patient
requires periodic infusion of
genetically engineered lymphocytes because : Show Answer

Q629) Statements related to human Insulin are given below.
Which statement(s) is/are correct about genetically engineered Insulin?
(a) Pro-hormone insulin contain extra stretch of C-peptide
(b) A-peptide and B-peptide chains of insulin were produced separately
in E.coli, extracted and combined by creating disulphide bond between
them.
(c) Insulin used for treating Diabetes was extracted from Cattles and
Pigs.
(d) Pro-hormone Insulin needs to be processed for converting into a
mature and functional hormone.
(e) Some patients develop allergic reactions to the foreign insulin.
Choose the most appropriate answer from the options given below Show Answer

Q630) When gene targetting involving gene amplification is attempted in an
individual's tissue to treat disease, it is known as : Show Answer

Q631) With regard to insulin choose correct options.
(a) C-peptide is not present in mature insulin.
(b) The insulin produced by rDNA technology has C-peptide.
(c) The pro-insulin has C-peptide
(d) A-peptide and B-peptide of insulin are interconnected by disulphide
bridges.
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Q632) Which of the following statements is not correct? Show Answer

Q633) Exploring molecular, genetic and species-level diversity for products of economic importance is called
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Q634) Identify the correct sequence of steps in each cycle of Polymerase Chain Reaction:
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Q635) Arrange the following steps of somatic hybridisation in a correct sequence.
A. Digestion of cell walls.
B. Isolation of naked protoplasts.
C. Fusion of protoplasts to get hybrid protoplast.
D. Isolation of single cells from two different varieites of plants.
E. Growing of hybrid protoplast to form a new plant.
Choose the correct answer from the options given below:
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Q636) Which of the following statements are not true regarding restriction endonucleases?
A. They are called molecular scissors.
B. These are the enzymes responsible for restricting the growth of bacteriophages in E. coli.
C. They cut the DNA only at the centre of the palindromic sites.
D. They remove nucleotides only from the ends of DNA fragments.
E. They recognise specific palindromic base-pair sequences.
Choose the answer from the options given below:
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Q637) Which of the following statements are correct with respect to DNA separation, isolation and visualization?
A. The cutting of DNA is done by molecular scissors.
B. The DNA fragments separate according to their size in an agarose gel, upon electrophoresis.
C. The separated DNA fragments can be seen without staining when exposed to UV light.
D. The separated DNA fragments, when stained with ethidium bromide, can be seen in visible light.
Choose the correct answer from the options given below:
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Q638) Insertion of a foreign DNA at BamHI site in an E.coli cloning vector pBR322 results in the loss of antibiotic resistance towards:
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Q639) The human protein named a-1-antitrypsin, obtained from transgenic animals, is used for the treatment of ___
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Q640) The toxin proteins isolated from Bacillus thuringiensis, coded by which of the following genes would contri cotton bollworms and corn borer, respectively?
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